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Functional expression of B7H1 on pancreatic carcinoma panc-1 cells

WU Qing-song1,2,HUANG Dong-sheng1,LIU Jun-wei1,JIN Hong-chuan3,SHEN Guo-liang1(1Department of General Surgery,2Key Laboratory of Biotherapy of Zhejiang Province,3Biomedical Research Center,Sir Run Run Shaw Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310016,China)  
AIM:To observe the effect of B7H1 expression in pancreatic carcinoma cells on the proliferation and activation of co-cultured T lymphocytes.METHODS: B7H1 expression in panc-1 cells before and after interferon-γ(IFN-γ) treatment or B7H1-siRNA transfection was evaluated by RT-PCR and flow cytometry.The influence of B7H1 expression on co-cultured PHA-activated T lymphocytes was determined by the methods of MTT and enzyme-linked immunosorbent assay(ELISA).RESULTS: B7H1 was highly expressed in panc-1 cells and up-regulated after IFN-γ stimulation.Such up-regulation led to the significant inhibition of T cell proliferation and secretion of cytokines such as IFN-γ and interleukin-2(IL-2).However,IL-10 production was enhanced.In contrast,knockdown of B7H1 expression in panc-1 cells by RNA interference resulted in increased T cell proliferation as well as IFN-γ and IL-2 production.Meanwhile,the IL-10 secretion decreased.CONCLUSION: B7H1-expressing panc-1 cells suppress T cell function by inhibiting T cell proliferation and production of Th1 cytokines.Suppression of B7H1 expression through siRNA restores T cell immune functions,indicating a potential strategy for immunotherapy against pancreatic cancer.
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