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《Chinese Journal of Pathophysiology》 2011-10
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Paeoniflorin attenuates LPS-induced acute lung injury in mice

ZHANG Bin1,LI Hong-mei2,WANG Yuan2,WANG Fa-qiang2,WANG Yi-yang2,Lü Xiu-xiu2,QI Ren-bin2,LU Da-xiang2,WANG Hua-dong2(1Department of Cardiothoracic Surgery,The Fifth Affiliated Hospital of Sun Yat-sen University,Zhuhai 519000,China;2Department of Pathophysiology,Key Laboratory of State Administration of Traditional Chinese Medicine,School of Medicine,Jinan University,Guangzhou 510632,China.)  
AIM:To investigate the mechanisms by which paeoniflorin(Pae) attenuates lipopolysaccharide(LPS)-induced acute lung injury in mice.METHODS: Male BALB/c mice were randomly divided into 4 groups: control,LPS,Pae+LPS,and Pae.Mice were administered intragastrically with double distilled water or Pae(20 mg/kg) once a day for 3 days.One hour after intragastrical treatment on the third day,LPS(20 mg/kg) or normal saline was injected intraperitoneally.Twelve hours after LPS challenge,the histological changes of the lung were observed,and histology score was also assessed.The myeloperoxidase(MPO),cytosolic phospholipase A2(cPLA2) and phosphorylated cytosolic phospholipase A2(phospho-cPLA2) in lung tissues were detected by Western blotting.RESULTS: LPS challenge resulted in acute lung injury,activated cPLA2 and increased MPO content in lung.Pretreatment with paeoniflorin significantly attenuated lung injury induced by intraperitoneal injection of LPS.The levels of MPO and phospho-cPLA2 in the lung tissues of the mice in Pae+LPS group were lower than those in LPS group(P0.05).CONCLUSION: Pretreatment with paeoniflorin remarkably reduces LPS-induced acute lung injury through inhibiting phosphorylation of cPLA2 and decreasing neutrophil infiltration in the lung.These findings provide a new strategy for the prevention and treatment of LPS-induced acute lung injury.
【Fund】: 国家中医药管理局病理生理学重点实验室开放基金资助项目;; 暨南大学“211工程”三期预研项目
【CateGory Index】: R285.5
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