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《Chinese Journal of Pathophysiology》 2012-02
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Effects of hypoxia in different degrees on in vitro invasion and migration of human hepatocarcinoma HepG2 cells

YU Gui-fang1,YAN Yue-hong1,WANG Rui-xin1,ZENG Wen-ting2,ZHU Ke-lun2(1The First Department of Internal Medicine,The Fifth Affiliated Hospital of Guangzhou Medical College,Guangzhou 510700,China;2Institute of Hepatology,The First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,China.)  
AIM: To study the effects and the underlying mechanisms of hypoxia on adhesion,invasion and migration of HepG2 human liver cancer cells cultured in vitro. METHODS: HepG2 cells were cultured under different concentrations of oxygen for 24 h.MTT assay was used to measure the cell adhesion rate.The Transwell chambers were used to assess in vitro invasion and migration.The expression of CD44v6,matrix metalloproteinase 2(MMP-2),MMP-9,hypoxia-inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) was detected by the methods of immunocytochemistry,gelatin zymography and RT-PCR. RESULTS: Hypoxia,especially under 3% oxygen,significantly enhanced the adhesion rates of HepG2 cells,and increased the number of the cells migrated through Matrigel-coated chambers(P0.05).HepG2 cells treated with 3% and 5% oxygen increased the expression of CD44v6,MMP-9,MMP-2,HIF-1α and VEGF. CONCLUSION: Pretreatment with moderate hypoxia enhances in vitro adhesion,invasion and migration of HepG2 cells.Multiple molecules including CD44v6,MMP-2,MMP-9,HIF-1α and VEGF may be involved in the processes.
【Fund】: 广东省科技计划项目(No.2010B060900092);; 广州市卫生局中医药科研项目资助(No.2010A34)
【CateGory Index】: R735.7
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