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《Chinese Journal of Pathophysiology》 2012-05
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Inhibitory effect of decorin on human pterygium fibroblasts in vitro

ZHOU Qing1,GUO Xian-yin2,YANG Xiao-xi1,CHEN Jian1 (1Department of Ophthalmology,The First Affiliated Hospital,Jinan University,Guangzhou 510632,China;2Department of Ophthalmology,Shilong People's Hospital,Dongguan 523320,China)  
AIM: To investigate the effect of decorin(DCN) on the proliferation and apoptosis of human pterygium fibroblasts(HPF) in vitro,and to compare the effect of mitomycin C(MMC) in order to search for a new method to prevent the recurrence after pterygium surgery.METHODS: Human pterygium fiborblasts were isolated from the caudomedial part of pterygium tissues in pterygium patients and then cultured in vitro using tissue inoculation method.The cells were treated with DCN and MMC at concentrations of 0.01,0.1,1,5 and 10 mg/L.The morphological alterations of HPF were observed after 24 h,48 h or 72 h of treatment.MTT method was used to assay the effects of the 2 drugs at different doses after 12 h,24 h and 48 h on the proliferation of the cells.The expression of proliferating cell nuclear antigen(PCNA) in each group treated with different doses of DCN was detected by the method of immunohistochemistry after 48 h.The cell cycle distribution was determined by flow cytometry analysis.RESULTS: After administration of 10 mg/L DCN or 1 mg/L MMC for 12 h,the proliferation of HPF was significantly inhibited by both drugs in a dose-and time-dependent manner(P0.05).After treated with 1~10 mg/L DCN for 48 h,the percentage of HPF in G0/G1 phase was increased,while the percentage of HPF in G2/M phase and S phase(G2/M%+S%) was decreased after treated with 5~10mg/L DCN for 48 h(P0.05).The late-apoptotic cells were not found in DCN group and MMC group.DCN dose-dependently inhibited the expression of PCNA in HPF(P0.05).CONCLUSION: Decorin significantly inhibits the proliferation of HPF,and blocks the cells in G1 phase.
【Fund】: 国家自然科学青年基金资助项目(No.81100637)
【CateGory Index】: R777.33
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【References】
Chinese Journal Full-text Database 8 Hits
1 XING Bang-rong;CHEN Yu-xian;ZENG Hua;YANG Xiao-yan;LIANG Cai-qian;ZHOU Wei-xiong;KONG Qing-lei;HAN Jian-hua;ZHANG Yong-biao;Department of Emergency,The Third Affiliated Hospital of Sun Yat-sen University;Department of Orthopaedics,The Third Affiliated Hospital of Sun Yat-sen University;;Effect of decorin on proliferation and collagen type I synthesis of stiff knee joint synovial type B cells[J];中国病理生理杂志;2015-07
2 DENG Shou-Heng;LI Lin-Jun;CAO Feng-Jun;Center of Oncology,People's Hospital Affiliated to Hubei University of Medicine;;Expression and significance of Decorin in two human endometrial carcinoma cells cultured in vitro[J];中国老年学杂志;2015-05
3 Gu Qiuyan;Dong Zefei;Fan Yamin;Yang Hongxi;Cui Junxia;Xingtai Medical College;;Study on the expression of Rho C protein in pterygium and its relationship with clinical pathological parameters[J];现代中西医结合杂志;2014-33
4 Gu Qiuyan;Xingtai Medical College;;Study on the expression and correlation of RhoC and MMP-9 in pterygium specimens[J];现代中西医结合杂志;2014-24
5 Cai Xiaojun;Zhang Lin;Department of Oncology,the Affiliated People's Hospital,Hubei University of Medicine;;The effect of transmembrane tumor necrosis factor-alpha on human cervical carcinoma HeLa cells[J];中国医师进修杂志;2014-09
6 CAI Xiaojun;CHEN Ping;CAO Fengjun;LI Linjun;LI Fang;DENG Shouheng;Oncology Center,Shiyan People's Hospital;;Expression and significance of decorin in Ishikawa cells[J];实用临床医药杂志;2014-05
7 Fu-Lu Zhong, Xue-Xi Li, Wei-Jie He Department of Ophthalmology, the 180th Hospital of Chinese PLA, Quanzhou 362000, Fujian Province, China;Sliding transplantation of auto-corneal limbus stem cell combined with mitomycin C for pterygium treatment[J];国际眼科杂志;2013-09
8 @Li Jianhong$Department of Ophthal- mology,the Central Hospital of Yiwu!Yiwu,Zhejiang 322000,China;Analysis of the effect of pterygium treated by different clinical methods[J];中国基层医药;2013-14
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHEN Yu-dong~1,WANG Ying-fen~2,CHEN Jian-su~1,LI Zhi-jie~1,CHEN Xiao-lin~3(~1Department of Ophthalmology,~3Department of Functional Experiments,Medical College of Jinan University,Guangzhou 510632,China;~2Departmen of Ophthalmology,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,China);Effect of diterpenoid compound 5F of Peris semipinnata L on apoptosis of human pterygium body fibroblasts in vitro[J];中国病理生理杂志;2006-05
2 FENG Xiu-yan, ZHANG Zhi-gang, ZHAO Zhong-hua, CHEN Qi, GUO Muyi. Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China;Study on the inhibitory effect of decorin on rat mesangial cell growth and its signal transduction pathway[J];中华肾脏病杂志;2005-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Zhao Zhijun;Li Qiang;Liu Daozhong;Zhou Xin;Department for Endemic Disease Prevention and Control, Qinghai Institute for Endemic Disease Prevention and Control;;Progression of inflammatory factors IL-1β and TNF-α in Kashin-Beck disease[J];中华地方病学杂志;2018-09
2 Deng Jun;Fu Shijie;Zhang Lei;Zhang Shiyi;He Hao;Zhou Xin;Department of Bone and Joint,The Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou City,Sichuan Province;;Research progresses of the correlation between cytokines and clinical manifestations of osteoarthritis[J];实用疼痛学杂志;2017-01
3 ZHENG Chunsong;YE Hongzhi;LI Xihai;FU Changlong;WU Guangwen;CHEN Xingqiang;LIU Xianxiang;Academy of Integrated Medicine affiliated to Fujian University of Traditional Chinese Medicine;Fujian Key Laboratory of Integrated Medicine on Geriatrics;;Impact of Duhuo Jisheng Tang(独活寄生汤) medicated serum on expression of matrix metalloproteinase and cyclooxygenase 2 in degenerative articular chondrocytes induced by interleukin-1 beta[J];中医正骨;2015-12
4 WEI Ni;XUE Zhi-feng;CHEN Zi-jia;SHI Yun-hui;ZHU Yue-lan;;Effect of Bushen Tongluo Granule(补肾通络颗粒) on Serum Cytokine and Water Ratio of Articular Cartilage in Rats with Knee Osteoarthritis[J];风湿病与关节炎;2015-10
5 LUO Jia;GUAN Xuefeng;Liaoning University of Traditional Chinese Medicine;The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine;;The effect of single traditional Chinese herb on chondrocyte apoptosis in knee osteoarthritis[J];中国骨质疏松杂志;2015-10
6 CHEN Mengfei;JIANG Hong;YU Zhenghan;Department of Chinese Traditional Traumatology, North Region of Suzhou Municipal Hospital;Orthopedics and Traumatology,Traditional Chinese Medicine Hospital of Suzhou City;;Effect of Duhuo Jisheng Decoction plus Astragalus on mRNA of Degenerative Bone Synoviocyte TNF-αand IL-6[J];四川中医;2015-10
7 LI Er-hong;SU Hou-heng;LI Guang-wen;MA Hong;LIU Wei-lin;Department of Rheumatology, Affiliated Qingdao Municipal Hospital of Qingdao university Medical College;;Influence of Uric Acid on Cell Viability and Expression of Inflammatory Factors of Chondrocytes[J];现代生物医学进展;2015-21
8 CHEN Wen-chao;ZHOU Ran;WANG Yong-hui;LI Yan-yan;CHAI Zhi;GAO Li;Shanxi University of Traditional Chinese Medicine;;Effect of Guling Paste and Its Split Reagent Preparations on IL- 18 and IL- 17 of Rat Model with Osteoarthritis[J];世界中西医结合杂志;2015-05
9 LI Min;LI Ge;JIA Huanhuan;WU Yue;TANG Lu;LU Xingyan;CHEN Jun;YANG Guozhu;ZHANG Yu;LI Qingnan;School of Life Science and Biopharmacology,Guangdong Pharmaceutical University;Guangdong Key Laboratory of Laboratory Animals,Guangdong Laboratory Animal Monitoring Institute;;Effects of different concentrations of hTNFα on chondrocytes and matrix-associated gene expression[J];中国骨质疏松杂志;2015-05
10 ZHENG Gongsheng;CHEN Chengran;HE Dingfeng;YAO Enfeng;HUANG Yuexia;XU Juhong;WANG Danna;Department of Orthopedics,Yuhuan Traditional Chinese Medicine Hospital in Zhejiang Province;Department of Clinical Laboratory,Luqiao Hospital of Taizhou Enze Medical Center(Group) in Zhejiang Province;;Influence and curative effect observation of sodium hyaluronate on serum matrix metalloproteinase-9,12 and 13 levels of patients with knee osteoarthritis[J];中国现代医生;2015-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 MA Yu-feng;QI Yin-ze;WANG Qing-fu;CHEN Zhao-jun;YU Dong;ZHENG Hao-yun;WU Ji;YIN Yue-shan;QI Qing-xue;The Third Hospital Affiliated to Beijing University of Chinese Medicine;;Progress on establishment of animal model of osteoarthritis by intra-articular injection[J];中国骨伤;2015-01
2 YANG Qian-jiao;YU Jin-di;PAN De-si;SHAN Song;LI Zhi-bin;NING Zhi-qiang;LU Xian-ping;Shenzhen Chipscreen Biosciences Ltd.;;Advance in research of selective JAK inhibitors for treating rheumatoid arthritis[J];中国新药杂志;2015-01
3 Thomas Mabey;Sittisak Honsawek;Department of Biochemistry,Faculty of Medicine,Chulalongkorn University,King Chulalongkorn Memorial Hospital,Thai Red Cross Society;;Cytokines as biochemical markers for knee osteoarthritis[J];World Journal of Orthopedics;2015-01
4 XIA Hong;HUANG Jiaqiang;MAO Fuqing;PENG Ke;HU Xu;Department of Orthopaedics,Central Hospital of Xiangtan City;Department of Clinical Laboratory,Central Hospital of Xiangtan City;Department of Emergency,Central Hospital of Xiangtan City;;Activity of cathepsin D and alpha-1 antitrypsin in patients with hip or knee osteoarthritis[J];中南大学学报(医学版);2014-11
5 ;Pharmacological effects and research advances of Baishao[J];中医临床研究;2014-29
6 ZHANG Zhi-Lin;XIAO Rong;LI Qing-Wei;College of Life Sciences,Liaoning Normal University;Key Laboratory of Liaoning Province for Biotechnology and Drug Discovery;;Diversified Functions of Cathepsin D[J];中国生物化学与分子生物学报;2014-07
7 GUO Cairong;XIONG Huiming;Clinical Laboratory,Taizhou Orthopedic Hospital;Clinical Laboratory,Luqiao Hospital of Taizhou Enze Medical Center (Group);;Changes of serum high sensitivity C-reactive protein, interleukin-1β, interleukin-6 and tumor necrosis factor-α(TNF-α) levels of patients with knee osteoarthritis before and after medical treatment[J];中国现代医生;2014-17
8 TIAN Xiao-jing;SHI Bing-wei;DING Zhi-xiang;QIAN Gao-chao;Department of Clinical Laboratory, Changzhou TCM Affiliated Hospital of Nanjing TCM University;;Clinical significance of IL-17 and IL-27 concentration in patients with activated rheumatoid arthritis[J];海南医学;2014-11
9 LAI Xiaofei;ZHANG Liping;Department of Laboratory Medicine, First Affiliated Hospital of Chongqing Medical University;;Correlative analysis of serum IL-27 with inflammatory markers and disease activity in patients with rheumatoid arthritis[J];免疫学杂志;2014-06
10 Wu Feixiang;Zhang Wei;Department of Orthopedics,Bethune Second Hospital Orthopaedic Hospital of Jilin University;;The update of interleukin in the pathogenesis of osteoarthritis[J];中华临床医师杂志(电子版);2014-05
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 GUO Xianyin;CAO Zheng;CHEN Shuo;ZHOU Peihui;Department of Ophthalmology,the Third People's Hospital;;The contrast of effects of pterygium excision combined with grafting auto conjunctiva and pterygium excision combined with biological amniotic membrane transplantation in treatment of pterygium.[J];中国医药科学;2015-20
2 Fan Guangxiang;Department of Ophthalmology,Zhengzhou Fifteenth People's Hospital;;Ocular surface reconstruction for treatment of 219 cases of pterygium[J];中华眼外伤职业眼病杂志;2014-08
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 FENG Xiu-yan, ZHANG Zhi-gang, ZHAO Zhong-hua, CHEN Qi, GUO Mu-yi. Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China;Influence on the expression of type I and type II transforming growth factor-β receptors in cultured rat mesangial cells transfected by decorin gene[J];中华肾脏病杂志;2005-05
2 WANG Hui-jun, ZHANG Zhi-gang, LIU Xue-guang, ZHANG Xiu-rong, CHEN Qi, GUO Mu-yi. Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China Corresponding author: GUO Mu-yi;The antagonistic effect on anti-thy-1 serum-induced nephritis of rats injected by decorin-transfected mesangial cells vector[J];中华病理学杂志;2003-05
3 WANG Wei-ming,CHEN Nan,LU Jian,CHEN Shi-shu,DONG De-chang. Department of Nephrology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China;Effect of decorin on the behavior of renal interstitial fibroblasts[J];中华肾脏病杂志;2003-05
4 WANG Hui-jun, ZHANG Zhi-gang, LIU Xue-guang, CHEN Guang-ping,CHEN Qi, GUO Mu-yi(Department of Pathology,Shanghai Medical College,Fudan University,Shanghia 200032,China);Influence on the Growth and Changes of Several Phenotypes of Cultured Rat MsC Transfected by DCN Gene[J];复旦学报(医学版);2003-05
5 CHEN Yu-dong(Department of Ophthalmology,Affiliated Hospital of Guangdong Medical College,Zhanjiang Guangdong 524001);The effect of diterpenoid compound 5F of Peris semipinnata L on the changes of the cell cycle and expressions of Ki-67 in fibroblasts of human pterygium in vitro[J];眼科;2002-05
6 WANG Hui jun,ZHANG Zhi gang,CHEN Guang ping △ ,LIN Wen sheng,CHEN Qi,GUO Mu yi (Department of Pathology,School of Basic Medical Sciences,Fudan University,Shanghai 200032; △Department of Radiation Oncology,North Shore LIJ Health System,;Constitution of Rat Glomerular Mesangial Cell Stably Expressing Decorin Gene[J];复旦学报(医学科学版);2001-02
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