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《Chinese Journal of Pathophysiology》 2013-04
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Gastrin promotes the migration and invasion of gastric cancer cells

LIU Jun1,ZHOU Jian-jiang1,2,ZHAO Yan1,XIE Yuan1(1Key Laboratory of Molecular Biology,2 Clinical Laboratory of Affiliated Hospital,Guiyang Medical College,Guiyang 550004,China)  
AIM: To study the effects of gastrin on the migration and invasion of gastric cancer cells in vitro.METHODS: The migration and invasion of gastric cancer AGS and SGC-7901 cells after treated with gastrin at concentrations of 10 nmol/L and 100 nmol/L were studied by wound-healing assay and Transwell migration and invasion assay.The cell proliferation was analyzed by MTT colorimetric method.The concentration of matrix metalloproteinase 2(MMP-2) in the culture medium was detected by ELISA.The AGS and SGC-7901 cells without treating with gastrin served as control cells.RESULTS: Compared with the control cells,the migration and invasion of AGS cells and SGC-7901 cells were significantly increased after treated with gastrin at concentrations of 10 nmol/L and 100 nmol/L.In control,10 nmol/L gastrin and 100 nmol/L gastrin groups,the mean numbers of the migrating cells were 56.0,88.1 and 106.4/view in AGS cells and 52.8,91.0 and 113.3/view in SGC-7901 cells,and the mean numbers of the invasive cells were 78.4,118.7 and 141.6/view in AGS cells and 87.3,124.6 and 147.4/view in SGC-7901 cells,respectively.The numbers of the migrating cells and invasive cells in 100 nmol/L gastrin group were higher than those in 10 nmol/L gastrin group.The cell proliferation rate and the concentration of MMP-2 in the culture medium in gastrin treatment groups were higher than those in control group.CONCLUSION: Gastrin promotes the migration and invasion of gastric cancer cells in a dose-dependent manner by increasing the MMP-2 secretion,which may be the key mechanism in the proliferation,invasion and metastasis of the cancer cells in vivo.
【Fund】: 国家自然科学基金资助项目(No.31060122);; 贵州省科技攻关计划基金资助项目(No.黔科合SY字[2011]3067号);; 贵州省科学技术基金资助项目(No.黔科合J字[2012]2039号)
【CateGory Index】: R735.2
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