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《Chinese Journal of Pathophysiology》 2013-08
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Protein kinase A regulates small-conductance calcium-activated potassium type 2 channel in chronic atrial fibrillation patients

ZHANG Li;LI Tao;LI Miao-ling;MAO Liang;TAN Xiao-qiu;YANG Yan;ZENG Xiao-rong;Key Laboratory of Ministry of Education for Medical Electrophysiology,Institute of Cardiovasology,Luzhou Medical College;  
AIM: To investigate the alteration of small-conductance calcium-activated potassium type 2(SK2) channel currents in atrial myocytes from atrial fibrillation(AF) patients,and the relationship between protein kinase A(PKA) and SK2 channel.METHODS: Right auricular tissues were obtained from the patients undergoing open-heart surgery with extracorporeal circulation.Single atrial myocytes were isolated by modified enzymatic dissociation method.The SK2 channel currents in the isolated human atrial myocytes were recorded using whole-cell patch-clamp technique.The alteration of SK2 channel currents and the regulation of SK2 channel by PKA were compared between sinus rhythm(SR) group and AF group.The total protein and PKA levels in human atrial tissues were detected by BCA assay and ELISA,respectively.RESULTS: The SK2 channel current densities and the proportion of SK2 channel currents in the integrated inward currents were significantly increased in AF group(all P 0.05 vs SR group).PKA-selective inhibitor H-89 reduced SK2 channel current densities and the proportion of SK2 channel currents in the integrated inward currents in both SR and AF groups,with larger reduction in AF group(all P 0.05 vs SR group).The PKA level was significantly decreased in AF atrial tissues(P 0.05 vs SR group).CONCLUSION: The increase in SK2 channel currents underlies the occurrence and maintenance of AF.PKA-dependent regulation may be involved in the remodeling of SK2 channel in both SR and AF human atrial myocytes,with a more powerful effect in AF.
【Fund】: 国家自然科学基金资助项目(No.30870903);; 四川省科技厅支撑计划项目(No.2011FZ060)
【CateGory Index】: R541.75
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Chinese Journal Full-text Database 3 Hits
1 LI Miao-Ling ZENG Xiao-Rong~* YANG Yan LIU Zhi-Fei DING Yin-Yuan ZHOU Wen PEI Jie Department of Myocardial Electrophysiology,Luzhou Medical College,Luzhou 646000,China;An improved method for isolation of single atrial myocyte from human heart[J];Acta Physiologica Sinica;2007-06
2 KE Qin-mei1, DU Yi-mei2,FENG Yi-bai1, TANG Ming2, DI Jiu-fang3 (1 Department of Geriatrics, The Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;2Department of Physiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;3 Department of Anesthesiology, Tengzhou Hospital of Traditional Chinese Medicine, Tengzhou 277500, China);Effect of adrenomedullin on L-type calcium currents and its signaling transduction process in guinea-pig ventricular myocytes[J];Chinese Journal of Pathophysiology;2004-04
3 HUANG Bing1, CHEN Lei2, WU Xue-ren2, SUN Li-zhong3, DONG Chao3, LIU Zhen-wei4, LI Li-huan2 (~ 1 Department of Anesthesiology, Beijing Hospital, Ministry of Public Health, Beijing 100730, China; ?2?Department of Anesthesiology;?3?Department of Cardiovascular Surgery, Fuwai Hospital & Cardiovascular Institute, CAMS & PUMC, Beijing 100037, China;?4?Institute of Pharmacology & Toxicology,Academy of Military Medical Sciences, Beijing 100850, China);The altered transient outward (I_(to1)) and ultra-rapid delayed rectifier (I_(Kur)) K~+ currents in right atrial myocytes of human atrial fibrillation[J];Chinese Journal of Pathophysiology;2005-01
Chinese Journal Full-text Database 10 Hits
1 Zhao Chaoren,Zhou Huafu,Feng Xun,Li Yi,Qian Jun,Xu Xianzeng.(Department of Cardiothoracic Suegery,the First Hospital Affiliated of Guangxi Medical University,Nanning 530021,China);THE DIVERSIFICATION OF ATRIAL MYOCYTEIN ACTION POTENTIAL IN PATIENTS WITH ATRIAL FIBRILLATION[J];Journal of Guangxi Medical University;2011-01
2 ZHANG Ying,DONG DeLi & YANG BaoFeng* Department of Pharmacology,State-Province Key Laboratories of Biomedicine-Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education,Harbin 150086,China;Atrial remodeling in atrial fibrillation and association between microRNA network and atrial fibrillation[J];中国科学:生命科学(英文版);2011-12
3 CHEN Li1,LI Zhong-wen2,YANG Yan3,LIU Zhi-fei3,LI Qi-yong4,ZENG Xiao-rong3(1.Department of Physiology,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041;2.Departmentof Physiology,Anhui Medicine University,Hefei 230061;3.Institute of Myocardial Electrophysiology,Luzhou Medicine College,Luzhou 646000;4.Cardiovascular Department,People's Hospital of Sichuan Province,Chengdu 610072,China);Change of Na~+ channel in chronic human atrial fibrillation[J];Basic & Clinical Medicine;2008-10
4 Lei Ming,et al Institute of Myocardial Electrophysiology,Luzhou Medical College;THE ESTABLISHMENT OF AN INTRACELLULAR FREE CA~(2+) CONCENTRATION MEASURING SYSTEM[J];Journal of Luzhou Medical College;2010-05
5 Li Tao,Li Miaoling,Lei Ming,Tan Xiaoqiu,Ding Yinyuan,Yang Yan,Zeng XiaorongDepartment of Electrophysiology,Institute of Cardiovasology,Luzhou Medical College;AN EFFECTIVE METHOD OF RECORDING SMALL CONDUCTANCE Ca~(2+)-ACTIVATED K~+ CHANNELS IN HUMAN ATRIAL MYOCYTE[J];Journal of Luzhou Medical College;2011-01
6 Li Tao,et al Department of Electrophysiology,Institute of Cardiovasology,Luzhou Medical College;EFFECT OF SODIUM CONCENTRATION ON NA~+ CURRENT IN CULTURED CARDIOMYOCYTES OF SUCKLING MOUSE[J];Journal of Luzhou Medical College;2011-04
7 ZHANG Guang-Wei1,GU Tian-Xiang1,WANG Chun1,YU Lei1,WEN Ti2 1Department of Cardiac Surgery,the First Hospital of China Medical University,Shenyang 110001,China;2 Central Laboratory,the Fourth Hospital of China Medical University,Shenyang 110001,China;Two-way concentration-dependent effect of H_2O_2 on I_(Kur) and I_(Ca,L) in human atrial myocytes[J];Acta Physiologica Sinica;2008-06
8 XU Kai,ZHANG Jia-wei(Department of Clinical Medicine,Shuqing Medical College,Zhengzhou,Henan 450064,China);Ion-channel remodeling mechanism in atrial fibrillation[J];Modern Medicine & Health;2011-11
9 WEI Fang, DENG Yun-kun, LI Yong-nian;Changes of MMP1,9 snd TIMP1 expression in patients with rheumatic heart disease and atrial fibrillation[J];Chinese Journal of Pathophysiology;2006-07
10 WANG Hua,LI Tao,LEI Ming,LI Miao-ling,DING Yin-yuan,YANG Yan,ZENG Xiao-rongΔ(Key Laboratory of Medical Electrophysiology,Ministry of Education of China,Luzhou Medical College,Luzhou 646000,China);Application of recording SK2 current in human atrial myocytes by perforated patch clamp techniques with the mix of β-escin and amphotericin B[J];Chinese Journal of Applied Physiology;2012-03
China Proceedings of conference Full-text Database 1 Hits
1 Yuan Wenjun,;Report on Advances in Circulatory Physiology[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 LI Gui-Rong 1, CHEN Yi-Yue 2 (1. Institute of Cardiovascular Science and Medicine, the University of Hong Kong, Hong Kong; 2. Faculty of Pharmacy, Guangdong College of Pharmacy, Guangzhou 510224, China);Mechanisms of heterogeneous electrophysiology in heart and the relation to electropharmacology and toxicology[J];Chinese Journal of Pharmacology and Toxicology;2001-02
2 XU Ping 1, DAI Ai-guo 1, HUANG Zhi-zhuang 2,ZHOU Hou-de 3 (? 1?Department of Respiratory Internal Medicine, The Third Affiliated Hospital of Nanhua university, Hengyang 421900, China;? 2?Department of Pharmacology , Xiangya Medical College of Zhongnan university, Changsha 410005,China;? 3?Department of Incretion and Metabolism, The Second Affiliated Xiangya Hospital of Zhongnan university, Changsha 410012,China);The acute inhibitory effect of adrenomedullin on hypoxia-induced pulmonary hypertension in rats[J];Chinese Journal of Pathophysiology;2003-02
3 ZHANG Biao~1, ZENG Xiao-rong~2, YANG Yan~2, LIU Zhi-fei~2, TONG Lin~3 (~1Department of Physiology, Guangdong Medical College, Zhanjiang 524023, China;~2Institute of Myocardial Electrophysiology, Luzhou Medical College, Luzhou 646000, China;~3The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China);Acetylcholine sensitive K~+ channel (K_(ACh)) in chronic human atrial fibrillation[J];Chinese Journal of Pathophysiology;2006-04
4 WEI Ying-Jie,ZHU Yan-Qing,CAO Yi-Wen,FU Ai-Hua,CHANG Zhao-Kang,TANG Jian Institute of Cardiovascular Research,Beijing Medical University,Beijing(100083);Distribution of adrenomedullin in cardiovascular system and its effect on cardiovascular function[J];CHINESE JOURNAL OF PATHOPHYSIOLOGY;1999-03
5 ZHANG Yu, ZENG Xiao-rong, YANG Yan, ZHANG Biao, LIU Zhi-fei, LI Miao-ling, ZHOU Wen, PE1 Jie. Institute of Myocardium Electrophysiology, Luzhou Medical College, Luzhou 646000,Sichuan, China;Human inward rectifying potassium current and Kir2.1 mRNA expression in myocytes isolated from patients with chronic atrial fibrillation[J];Chinese Journal of Cardiology;2006-01
6 LI Miao-ling, ZENG Xiao-rong, YANG Yan, LIU Zhi-fei,ZHOU Wen,DING Yin-yuan, PEI Jie. Department of Myocardial Electrophysiology, Institute of Cardiovascular Research, Luzhou Medical College, Luzhou 646000, China;Research of L-type Ca~(2+) channel current in atrial myocytes of patients with chronic atrial fibrillation[J];Chinese Journal of Cardiology;2006-04
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