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《Chinese Journal of Pathophysiology》 2013-11
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Interferon-γ up-regulates programmed death ligand 1 in human gastric cancer cells

CHEN Shu-fen;HOU Jing-ying;WU Shu-yun;WANG Ling-yun;Department of Gastroenterology,Sun Yat-sen Memorial Hospital of Sun Yat-sen University;Emergency Department,Sun Yat-sen Memorial Hospital of Sun Yat-sen University;  
AIM: To study the expression of programmed death ligand 1( PD-L1) in human gastric cancer cells with or without the stimulation of interferon-γ( IFN-γ). METHODS: The protein levels of PD-L1 in 4 different human gastric cancer cell lines AGS,BGC823,MGC803 and SGC7901 with or without IFN-γ treatment were analyzed by flow cytometry. The mRNA expression of PD-L1 in those cell lines was also detected by real-time PCR. RESULTS: Flow cytometry analysis showed that the rates of PD-L1 surface expression in the 4 human gastric cancer cell lines AGS,BGC823,MGC803 and SGC7901 were( 1. 567 ± 0. 109) %,( 2. 640 ± 0. 577) %,( 1. 760 ± 0. 236) % and( 16. 030 ± 1. 289) %,respectively. After the 4 gastric cancer cell lines were treated with IFN-γ at different concentrations or for different time,the PD-L1 surface expression increased at different levels with significant differences between groups. Real-time PCR also indicated that IFN-γ up-regulated PD-L1 expression at mRNA level. CONCLUSION: PD-L1 surface expression is found in human gastric cancer cell lines AGS,BGC823,MGC803 and SGC7901. IFN-γ up-regulates the expression of PD-L1.SGC7901 cell line,which is from metastatic lymph nodes,expresses the highest protein level of PD-L1 among the 4 cell lines,indicating that PD-L1 expression may be related to lymph node metastasis,not to differentiation grade. IFN-γ may mediate the tumor immune escape so that it should be carefully applied in the treatment of gastric cancer.
【Fund】: 广东省科技社会发展项目(No.2012B031800362)
【CateGory Index】: R735.2
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