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《Chinese Journal of Pathophysiology》 2015-07
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Effect of decorin on proliferation and collagen type I synthesis of stiff knee joint synovial type B cells

XING Bang-rong;CHEN Yu-xian;ZENG Hua;YANG Xiao-yan;LIANG Cai-qian;ZHOU Wei-xiong;KONG Qing-lei;HAN Jian-hua;ZHANG Yong-biao;Department of Emergency,The Third Affiliated Hospital of Sun Yat-sen University;Department of Orthopaedics,The Third Affiliated Hospital of Sun Yat-sen University;  
AIM: To explore the effects of decorin on procollagen type I( Pc I),mRNA expression,collagen type I synthesis and proliferation of synovial type B cells of stiff knee joint synovial membrane. METHODS: Type B cells of synovial membrane were isolated from the stiff knee joint synovial membrane and cultured in vitro. The cells were treated with decorin at concentrations of 0. 1 mg / L,5 mg / L and 10 mg / L. After cultured for 24 h,48 h and 72 h,the cell proliferation rates were measured by MTT colorimetric determination. Cell cycle distribution and apoptosis were analyzed by flow cytometry. The mRNA level of Pc I was detected by RT-PCR,while collagen type I was measured by Western blot. RESULTS: The proliferation of synovial type B cells was significantly inhibited,the percentage of synovial type B cells at G1 phase was significantly increased by 5 mg / L and 10 mg / L decorin( P 0. 05),and PcⅠ mRNA expression and collagen type I synthesis were significantly decreased. The cells with late apoptosis were not found in control group and experimental groups. CONCLUSION: Recombinant human decorin inhibits synovial type B cell proliferation and decreases PcⅠ mRNA expression and collagen type I synthesis in synovial type B cells of stiff knee joint synovial membrane in vitro,suggesting that decorin potentially contributes to the therapy of human knee stiffness.
【Fund】: 广东省自然科学基金资助项目(No.S2013010015461)
【CateGory Index】: R684
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【Citations】
Chinese Journal Full-text Database 3 Hits
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【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 XING Bang-rong;CHEN Yu-xian;ZENG Hua;YANG Xiao-yan;LIANG Cai-qian;ZHOU Wei-xiong;KONG Qing-lei;HAN Jian-hua;ZHANG Yong-biao;Department of Emergency,The Third Affiliated Hospital of Sun Yat-sen University;Department of Orthopaedics,The Third Affiliated Hospital of Sun Yat-sen University;;Effect of decorin on proliferation and collagen type I synthesis of stiff knee joint synovial type B cells[J];中国病理生理杂志;2015-07
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