Determination of prim-O-glucosylcimifugin, 4'-O-β-D-glucosyl-5-O-methylvisamminol,notopterol,isoimperatorin and asarinin in Jiulong Huafeng Pills by HPLC

GAO Sen;LI Zheng-xiang;WANG Zhi-tao;FANG Zhi-zhong;General Hospital,Tianjin Medical University;Department of Pharmacy,College of Pharmacy,Tianjin Medical University,Tianjin Key Laboratary on Technologies Enabling Development of Clinical Therapeutics and Diagnostics ( Theranostics);  

AIM To develop an HPLC method for determining the contents of prim-O-glucosylcimifugin,4'-O-β-D-glucosyl-5-O-meth-ylvisammioside,notopterol,isoimperatorin and asarinin in Jiulong Huafeng Pills( Saposhnikoviae Radix,Notopterygh Rhizoma et Radix,Asari Radix et Rhizoma,Rhei Radix et Rhizoma,Platycodonis Radix,etc.). METHODS An Hypersil C18column was used as the chromatographic column,the flow rate was 0. 9 mL / min. For prim-O-glucosylcimifugin and 4'-O-β-D-glucosyl-5-O-methylvisamminol,the mobile phase A consisted of methanol-acetonitrile( 2 ∶ 1),the mobile phase B consisted of 0. 5% phosphoric acid solution,UV detection wavelength was set at 254 nm. For notopterol and isoimperatorin,the mobile phase A consisted of acetonitrile,the mobile phase B consisted of 0. 1% acetic acid solution,the detection wavelength was set at 310 nm. For asarinin,the mobile phase consisted of acetonitrile-methanol( 85 ∶ 15),the UV detection wavelength was set at 286 nm. RESULTS There was a good linear relationship between the concentration of prim-O-glucosylcimifugin,4'-O-β-D-glucosyl-5-O-methylvisamminol and peak area value when the concentrations of prim-O-glucosylcimifugin and 4'-O-β-D-glucosyl-5-O-meth-ylvisammioside were within the range of 0. 014 6- 0. 292 0 μg( r = 0. 999 1),0. 010 8- 0. 216 0 μg( r = 0. 999 3). The average recoveries were 97. 55%( RSD = 1. 46%) and 97. 73%( RSD = 0. 1. 18%). There was a good linear relationship between the concentration of notopterol,isoimperatorin and peak area value when the concentrations of notopterol and isoimperatorin were within the range of 0. 017 4- 0. 348 0 μg( r = 0. 999 3),0. 012 8- 0. 256 0 μg( r = 0. 999 7). The average recoveries were 97. 62%( RSD = 1. 43%) and 98. 11%( RSD = 1. 39%). There was a good linear relationship between the concentration of asarinin and peak area value when the concentrations of asarinin was within the range of 0. 012 4-0. 248 0 μg( r = 0. 999 2). The average recovery was 97. 65%( RSD = 1. 24%). CONCLUSION The method is accurate,sensitive,reproducible and may be used in the determination of prim-O-glucosylcimifugin,4'-O-β-D-glucosyl-5-O-methylvisamminol,notopterol,isoimperatorin and asarinin in Jiulong Huafeng Pills.

【CateGory Index】： R284.1

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