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《Chinese Traditional and Herbal Drugs》 2002-05
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RAPD analysis of genetic polymorphism and molecular identification of Citrus medica cv. sarcodactylis from Jinhua

MA Bo jun, ZHANG Bin yi, CHEN Biao, CHEN Bing chu (Academy of Life and Environment Sciences, Zhejiang Normal University, Jinhua Zhejiang 321004, China)  
Object To analyse genetic polymorphism in DNA level of three species and determine them in molecular level by using of the optimization of Citrus medica cv. sarcodactylis (Noot.) Swingle RAPD reaction.Methods RAPD reactions were performed under the following conditions:template DNA 10~100 ng/μL, 3.0~5.0 mmol/L MgCl 2, 200 μmol/L dNTP,0.2 μmol/L 10 mer Primer, lunit Taq Polymerase/25 μL reaction volume.Results Twelve efficient and stable primers were selected from 38 primers. Phenograms were constructed by using genetic distance UPGMA method. Based on the results of the RAPD analysis and on a review of morphological characterisitic, C. medica cv. sarcodactylis species BAIHUAQINGPI could be used as BAIHUABAIPI in three species, there were some special amplified bands in every RAPD reactions from different species. Conclusion This method could serve as an easy and highly efficient method for C. medica cv. sarcodactylis genetic polymorphism and molecular identification of species.
【Fund】: 浙江省自然科学基金项目资助 (3 0 10 2 6);; 浙江省大型仪器测试基金项目资助 (0 0 0 70 )
【CateGory Index】: R282.21
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