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《Chinese Traditional and Herbal Drugs》 2003-09
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Effect of plant growth regulators on several characteristics during in vitrobud regeneration of Polygonatum cyrtonem

a XU Hong-mei 1, ZHAO Dong-li 2 (1 Editorial Office of Journal, Beijing Agricultura l College, Beijing 102206, China; 2 Medical College of Dalian University, Dalian 116622, China)  
Object To study the effects of plant growth regu la tors (PGR) with various combinations on several characteristics including number s o f per explant, production frequencies of leaves, abiogenesis of root and rhizome during in vitro bud regeneration process of Polygonatum cyrtonema Hua so as to search for the optimum in vitro regenera tion process for P. cyrtonema. Methods Granul ar calli were induced on MS+BA 1 0 mg/L+ 2, 4-D 0.5 mg/L by bud explant cuttings of P. cyrtonema. The ca lli were inoculated into MS media with various combined PGR. The characte ristics described above were observed and the effects of various combined PGR we ne obtained by statistics in two months R esults Among various combined PGR, TDZ 1.5 mg/L+2, 4-D 1.0 mg/L showed the best effect for the proliferation of buds, BA 1.0-2.0 mg/L+NAA 1 .0-2 .0 mg/L for improving of leave development, BA 2.0 mg/L+NAA 1.0 mg/L for root pr oduction and BA 2.0 mg/L+2, 4-D 1.0-2.0 mg/L for rhizome growth. The number of buds per explant was up to 8.6 in average, and the production rates of leaves, r oot, and rhizome over 0.5 cm in diameter were up to 95%, 51.9% and exceeding 100 %, respectively However, the medium containing only auxin such as NAA, IAA, IB A or 2, 4-D with 0.5-1.0 mg/L is much better for root induction of new buds tha n the original media with BA 2.0 mg/L+NAA 1.0 mg/L on which the explants can nat urally root. Conclusion The in vitro regener ation process of P. cyrtonema can be achieved by the following s teps: Firstly, proliferation of buds can be realized on medium with TDZ 1.5 mg/L +2, 4-D 1.0 mg/L. Then, normal leaves can be developed after the new buds have been transferred to medium with BA 1.0-2.0 mg/L+NAA 1.0-2.0 mg/L. Finally, vigor ous roots can be formed from regenerated buds on 1/2 MS medium with+ NAA, IAA, I BA or 2, 4-D 0.5-1.0 mg/L. Medium with BA 2.0 mg/L+2, 4-D 1.0-2.0 mg/L can be specially applied to improving the growth of rhizomes, the medicinal part of P. cyrtonema
【CateGory Index】: S567.2
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