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Molecular mechanism of Rehmannia glutinosa oligosaccharides on improvement of insulin resistance of HepG2 cell in vitro

ZHANG Ru-xue1,JIA Zheng-ping1,LI Mao-xing1,WANG Juan1,GUO Li-min2,ZHANG Xiao-hua1(1.Key Discipline of Clinical Chinese Materia Medica,State Administration of Traditional Chinese Medicine,Lanzhou General Hospital,Lanzhou Command,PLA,Lanzhou 730050,China;2.Disease Preventive and Control Center of Gansu Province,Lanzhou 730050 China)  
Objective To study the molecular mechanism of Rehmannia glutinosa oligosaccharides(ROS) on the improvement of insulin resistance of HepG2 cell in vitro.Methods The mRNA expression of peroxisome proliferator-activated receptor alpha(PPAR-α),insulin receptor(IR),GLUT4 and GLUT2 related to insulin sensitivity was detected by reverse transcription PCR(RT-PCR).Results The mRNA expression of PPAR-α,IR,and GLUT4 was up-regulated by ROS(10 mg/L),but the mRNA expression of GLUT2 was inhibited.Conclusion The improving effect of ROS on the improvement of insulin-resistance of HepG2 cell in vitro is related to the activation of PPAR-α and the regulation of mRNA gene expression of IR and GLUT4,as well as the inhibition of GLUT2 gene expression.
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