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《Chinese Traditional and Herbal Drugs》 2011-05
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Technology optimization for extracting total favonoids from Gynostemma pentaphyllum by cellulase

OUYANG Hui1,2,TIAN Qi-jian1,YU Ji1,LI Shun-xiang2 1.Key Laboratory of Plant Resource Conservation and Utilization,Jishou University,Jishou 416000,China 2.Key Laboratory of Modernization of Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China  
Objective To optimize the extracting process of total flavonoids from Gynostemma pentaphyllum.Methods The total flavonoids were extracted with ethanol 95% after the cellulose treatment to G.pentaphyllum,and the effects of enzyme dosage,enzymatic treatment temperature,enzymatic treatment time,and pH value on the total flavonoids yield was studied with response surface method.Results The optimal enzymatic extracting process of total flavonoids was as follows: enzyme dosage 120 U/mL,enzymatic temperature 49 ℃,enzymatic time 150 min,and pH value 3.9,and the total flavonoids yield reached 0.357%.Conclusion Cellulase is suitable for the total flavonoid extraction from G.pentaphyllum.
【Fund】: 湖南省科技计划项目(2009FJ3028)
【CateGory Index】: R284.2
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