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《Chinese Traditional and Herbal Drugs》 2011-10
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Effect of naphthalencacetic acid and 2,4-dicholorophenoxyacetic acid on callus cell ploidy and secondary metabolites of Glycyrrhiza uralensis

HUANG Hui-ying1,4,MA Wen-fang2,CHEN Xiao-Yan3,WANG Qing1,2 1.Gansu Key Laboratory of Crop Genetic Improvement and Germplasm Enhancement,Lanzhou 730070,China 2.College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China 3.College of Life Science,Lanzhou University,Lanzhou 730070,China 4.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China  
Objective To establish the system of high frequency chromosome doubling and to lay the fundation for obtaining the strain of licorice with a high active ingredient content.Methods Exogenous hormone of naphthalencacetic acid(NAA) and 2,4-dicholorophenoxyacetic acid(2,4-D) were used to induce callus of Glycyrrhiza uralensis explants from cotyledons,hypocotyls,and radicle,and the effect of NAA and 2,4-D were tested on callus cell ploidy and content of secondary metabolites.Results The result showed that there were significant effects of NAA and 2,4-D with different concentrations on cell ploidy of different explants callus.The frequency of cell with chromosome 2n 14 and 2n = 28 was significantly higher in hypocotyls callus than those in cotyledons and radicle.They were up to 42.38% and 25.19%,respectively.Meanwhile,the frequency of cell with 2n 14 and 2n = 28 in cotyledons,hypocotyls,and radicle callus all reached the highest under the concentration of 2.0 mg/L NAA,2.0 mg/L 2,4-D,and 1.0 mg/L 2,4-D,respectively.They were up to 61.50%,39.20%,63.00%,36.23%,63.50%,and 37.50%,respectively.The doubling rate of callus cell increased by the extension of cultured time.Furthermore,there were significant effects of NAA and 2,4-D on promoting the contents of glycyrrhizic acid and licorice flavanone.The contents of glycyrrhizic acid and licorice flavanone of cotyledons callus reached the highest on MS medium with 1.0 mg/L NAA and 1.0 mg/L 2,4-D,they were up to 52.13 mg/g and 8.36 mg/g,respectively.And the contents in hypocotyls callus reached the highest on MS medium with 2 mg/L NAA,they were up to 49.01 mg/g and 8.54 mg/g,respectively.Besides the contents of glycyrrhizic acid and licorice flavanone showed positive correlation with chromosome doubling frequency of callus cell.Conclusion There are significant effects of NAA and 2,4-D with different concentrations to promote chromosome doubling and the content increasing of glycyrrhizic acid and licorice flavanone in different explants callus.It suggests that perhaps the new material in licorice with higher pharmacodynamic components will be obtained by chromosome doubling.
【Fund】: 甘肃省自然科学基金资助项目(0803RJZA032)
【CateGory Index】: S567.71
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