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《Chinese Traditional and Herbal Drugs》 2015-22
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Polymorphism analysis of endangered Notopterygium incisum and endemic species based on cpDNA trnT-trnL sequences

YANG Lu-cun;LIU He-chun;ZHOU Xue-li;XU Wen-hua;ZHOU Guo-ying;Northwest Institute of Plateau Biology, Chinese Academy of Sciences;Key Laboratory of Tibetan Medicine Research, Chinese Academy of Sciences;University of Chinese Academy of Sciences;Tiebujia Grassland Improvement Experiment Station;  
Objective To analyze the genetic diversity and genetic structure of 245 plant samples in Notopterygium incisum from different regions. Methods Genetic diversity and genetic structure of 245 samples of N. incisum were studied using chloroplast cpDNA trnT-trnL direct sequencing method and Neighbor-joining(NJ) method. Results Haplotype diversity(Hd) and nucleotide diversity(Pi) at the species level were 0.873 and 0.004 07, respectively. At the population level, Hd ranged with 0.000—0.900, and Pi ranged with 0.000—0.054 4. It indicated that N. incisum had a moderate level of genetic diversity than N. franchetii plants of the genus Notopterygium Boiss. Molecular variance analysis(AMOVA) indicated that high genetic differentiation(77.55%) existed among populations, and gene flow was low(Nm = 0.145) among populations. Based on the NJ tree, the 31 wild populations tested were clustered into two groups. Conclusion N. incisum has the midium cp DNA diversity and higher population genetic differentiation.
【Fund】: 青海省青年基金项目:珍稀濒危药用植物羌活的濒危机制研究(2013-Z-942Q)
【CateGory Index】: S567.239
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