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《Chinese Journal of Otorhinolaryngology-Skull Base Surgery》 2008-03
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Study of the protection of adenovirus-mediated gene transfer of Bcl-2 to the primary cultured spiral ganglion cells agaist apoptosis induced by oxidative stress

LUO Ling-hui,GONG Shu-sheng,CHEN Pei(Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)  
Objective To investigate the effect of exogenous Bcl-2 protein expression on the apoptosis of the primary cultured spiral ganglion cells(SGC)induced by oxidative stress.Methods The spiral ganglion cells of newly born rats were cultured in vitro.The adenoviral vectors containing human Bcl-2 gene(group A)or no gene(group B)were used for infecting the rat SGCs.Then the SGCs were exposed to hydrogen peroxide at the concentration of 100 μmol/L.Cell nuclei were stained simultaneously with the DNA binding dyes Hoechst 33258 and propidium iodide.Results At the concentration of 100 μmol/L of hydrogen peroxide,apoptosis was the main reason for cell death.The number of the impaired SGCs in group A was larger than that in group B.Conclusion Bcl-2 gene can protect SGCs against the apoptosis induced by hydrogen peroxide.
【Fund】: 国家自然科学基金资助项目(30471879);; 高校青年教师奖;; 湖北省卫生厅科研基金资助项目(JXIBO56)
【CateGory Index】: R363
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【References】
Chinese Journal Full-text Database 1 Hits
1 Chen Qingguo*,Chu Hanqi,Wang Xianhong,Chen Jin,Li Jianling,Zhou Liangqiang,Xiong Hao,Wang Yan,Li Zhiyong,Cui Yonghua (*Department of Otorhinolaryngology Head and Neck Surgery,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China);The Growth Characteristic of Spiral Ganglion Cells from Newborn Rats and the Liposome-mediated EGFP Transfection Efficiency in Vitro[J];Journal of Audiology and Speech Pathology;2010-05
【Citations】
Chinese Journal Full-text Database 2 Hits
1 LUO Linghui GONG Shusheng SONG Peng Yan Kaisheng (Department of Otolaryngology, Union Hospital, Huazhong University of Science and Techno- logy , Wuhan, 430022, China);Experimental study of primary culture methods for spiral ganglion cells of newborn rats[J];Journal of Clinical Otorhinolaryngology;2006-04
2 LUO Ling-hui,GONG Shu-sheng, SONG Peng, YAN Kai-sheng,LIU Ying-peng. Department of Otorhinolaryngology, Union Hospital of Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022,China;Construction of recombinant adenovirus containing human Bcl-2 and its expression in the spiral ganglion cells[J];Chinese Journal of Otorhinolaryngology;2005-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHI Jinfeng1, XIA Yin1, LEI Li1, WANG Hong2, FAN Erzhong2 (1 Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China; 2 Beijing Institute of Otolaryngology, Beijing, 100005, China);The impact of different culture conditions on the spiral ganglion neurons in vitro[J];Chinese Archives of Otolaryngology-head and Neck Surgery;2007-01
2 LUO Linghui Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,430022,China GONG Shusheng Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Key Laboratory of Otolaryngology Head and Neck Surgery(Capital Medical University),Ministry of Education,Beijing,100730,China;Adenovirus-mediated gene transfer of Bcl-2 promote the survival of primary culture of spiral ganglion cells in vitro[J];Chinese Archives of Otolaryngology-Head and Neck Surgery;2008-08
3 DU Hai ke *,WANG Shi xin *,LIU Yan,GAO Xiu xia * ( * Affiliated Hospital of Medical College of Chinese Peoples Armed Polices Forces,Tianjin 300162 China);Effect of Niacin on Ⅰ and Ⅲ Collagen Expression in the Lung of Silica Exposed Rat[J];Industrial Health and Occupational Diseases;2004-02
4 LIU Tao,CHEN Yan-fang,LIU Yi,et al.(Department of Otolaryngology,West China Hospital,Sichuan University,Chengdu 610041,China);Primary Culture in Vitro and Identification of Murine Spiral Ganglion Neurons[J];West China Medical Journal;2008-01
5 LUO Linghui GONG Shusheng SONG Peng Yan Kaisheng (Department of Otolaryngology, Union Hospital, Huazhong University of Science and Techno- logy , Wuhan, 430022, China);Experimental study of primary culture methods for spiral ganglion cells of newborn rats[J];Journal of Clinical Otorhinolaryngology;2006-04
6 YAN Kaisheng XUE Qiuhong LUO Linghui GONG Shusheng (Department of Otolaryngology, Union Hospital, Huazhong University of Science and Techno- logy , Wuhan,430022, China);Excitotoxic effects of glutamate on the in vitro spiral ganglion cells[J];Journal of Clinical Otorhinolaryngology;2006-13
7 LUO Linghui1 GONG Shusheng2 CHEN Pei1(1Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China;2Department of Otolaryngology-Head and Neck Surgery,Tongren Hospital,Capital Medical University);A study of spiral ganglion cells death pathway induced by hydrogen peroxide[J];Journal of Clinical Otorhinolaryngology Head and Neck Surgery;2009-05
8 WANG Guopeng1 LUO Linghui2 XIE Jing1 CHEN Pei2 FU Yong3 ZHONG Gang2 GONG Shusheng1(1Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Beijing,100730,China;2Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology;3Department of Otolaryngology,the First Affiliated Hospital,College of Medicine,Zhejiang University);Comparison of lentivirus and adenovirus vector mediated gene transfer into cultured spiral ganglion cells[J];Journal of Clinical Otorhinolaryngology Head and Neck Surgery;2011-04
9 ZHAI Meng-yao1,GAO Zhi-qiang1,LIANG Ying-juan2,SHEN Peng1(1. Department of Otorhinolaryngology Head and Neck Surgery,Peking Union Medical College Hospital,Chinese Academy ofMedical Sciences and Peking Union Medical College; 2. Center of Virus Resources,Institute for Viral Disease Control andPrevention,Chinese Center for Disease Control and Prevention);Experimental Study on Methods for Primary Culture of Geniculate Ganglion Cells and Spiral Ganglion Cells of Adult Guinea Pigs[J];Journal of Capital Medical University;2009-06
10 MA Chao, CHEN Yong, GONG Li, et al (Lanzhou Institute of Biological Products, Lanzhou 730046, China);Construction and Packaging of Recombinant Adenovirus Vector Carrying gB Gene of Human Cytomegalovirus[J];Chinese Journal of Biologicals;2008-07
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LU Na1,LI Chao2,CHENG Yuan1,DU Ai-lin1 Department of Physiology and Neurobiology1,Department of Ophthalmology,Third Affiliated Hospital2,Xinxiang Medical College,Xinxiang 453003,China;Protective effects of progesterone against high intraocular pressure-induced retinal ischemia-reperfusion in rats[J];Journal of Southern Medical University;2008-11
2 LI Yu-de1,DUAN Xiang-xing1.QIAN Zhi-min2(1.Inner Mongolia Medical College,Huhhot 010059 China;2.Department of Ophthalmology,Inner Mongolia Hospital,Huhhot 010017 China);Effect of Ciliary Neurotrophic Factor on the Apoptosis of Retinal Ganglion Cells after Injury in Optic Nerve[J];Inner Mongolia Medical Journal;2009-05
3 Li Ke,Zhang Hong Department of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030;The Effects of Recombinant Human Erythropoietin on Apoptosis and BCL-2 Expression of Cultured Retinal Neurons under Pressure[J];Acta Medicinae Universitatis Scientiae et Technologiae Huazhong;2009-01
4 Hong Su~1,Su Liu~1,Zhigang Wang~2,Wenyue Xie~1,Bing Jiang~1,Haibo Xiong~1 1 Department of Ophthalmology,Second Hospital of Chongqing Medical University,Chongqing 400010,China 2 Institute of Ultrasonic Imaging,Chongqing Medical University,Chongqing 400010,China;In vivo transfection of enhanced green fluorescent protein in rat retinal ganglion cells mediated by ultrasound-induced microbubbles[J];中国神经再生研究(英文版);2009-06
5 ZHANG Zuo Ming,GU Yong Hao,GUO Qun,LONG Tan, LI Li,SHI Li,HOU Bao Ke From the Department of Clinical Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China;Guideline for visual electrophysiological testing experiment in mice and rat[J];Recent Advances In Ophthalmology;2004-02
6 SUN Gang,WANG Jing Hua From the Department of Ophthalmology,Affiliated Hospital of Qingdao Medical College,Qingdao 266012,Shandong Province,China;Glaucomatous damages in the central visual system[J];Recent Advances In Ophthalmology;2004-02
7 Li Tong; Liu Jianzong Department of Opthalmology, the Second Affiliated Hospital, Hebei Medical University , Shijiazhuang 050000;An experimental study on protective effects of flunarizine in retinal ischemic injury[J];Chinese Ophthalmic Research;2001-01
8 Li Yangjun, Yang Xinguang, Zhang Zuoming. Department of Ophthalmology, Tangdu Hospital, Forth Military Medical University,Xi' an 710038;The effects of Glutamate intravitreal injection on electroretinogramin adult rabbits[J];Chinese Ophthalmic Research;2002-03
9 Li Liangdong,Huang Zhihua,Li Xiao,Liu Dong,Zhan Minyan,Zeng Jing.Department of Physiology,Gannan Medical University,Ganzhou 341000,China;Effects of DSS on anti-oxidation and NOS activity after retinal ischemia/reperfusion injury in rats[J];Chinese Ophthalmic Research;2009-07
10 Li Yuehua,Ma Ke,Xu Liang.Department of Ophthalmology,Beijing Tongren Hospital,Beijing 100005,China;The neuroprotective effect of extact of ginko biloba on retinal ganglion cells after retinal ischemia-reperfusion[J];Chinese Ophthalmic Research;2009-08
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 SUN Wang-wu,CAO Jun-xin,SONG Xue-xiong(College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China);Studies on Transfection of Anti-swine Fever Virus Gene into Mouse Embryonic Fibroblasts (MEF) Mediated by LiPosome[J];China Animal Husbandry & Veterinary Medicine;2011-10
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