Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Antituberculosis》 2019-03
Add to Favorite Get Latest Update

MIRU-VNTR genotyping and clustering of Mycobacterium tuberculosis isolates in Yichang

LIU Xiao-jun;YU Feng-hua;YU Yun-fang;JI L-yu;ZHOU Pan;ZHAO Yan-lin;Clinical Laboratory, Yichang Center for Disease Control and Prevention,Hubei Province;  
Objective To determine the genotypes and clustering patterns of Mycobacterium tuberculosis in Yichang and provide evidence for use in the prevention and treatment of tuberculosis.Methods Sputum samples determined to be positive for Mycobacterium tuberculosis using gene microarrays were collected in Yichang from December 2016 to May 2017.After culturing in Roche medium, 367 Mycobacterium tuberculosis isolates were genotyped using the internationally recognised 24 locus MIRU-VNTR system, and the resolution and variation of the MIRU locus was evaluated using the Hunter-Gaston index(HGI) and genetic difference value(h).MIRU-VNTR results were analyzed using BioNumerics 5.0 software to calculate recent transmission rates.The study population of 367 strains, isolated from 367 patients, was subdivided into 321 strains isolated from new TB cases and 46 strains from relapsed cases.Clustering rates were analysed separately for each set of strains.The clustering rates for new and retreated cases were compared using the chi-square test.P 0.05 was considered statistically significant.Results Genotyping of the 367 M.tuberculosis strains using the 24 locus MIRU-VNTR system gave an HGI value of 0.999.The locus with the highest genetic diversity was QUBllb, with an h value of 0.79, and that with the lowest genetic diversity was MIRU24, with an h value of 0.03.A total of 324 genotypes were identified, polymorphisms being evident.Results from clustering analysis indicated that 90 strains formed 39 clusters, with a clustering rate of 24.52%(90/367) and the recent transmission rate of 13.90%(51/367).The clustering rate of strains from relapsed cases(21.74%, 10/46) was not significantly different from that for strains isolated from new cases(24.92%, 80/321)(χ~2=0.220, P =0.639).Conclusion Tuberculosis cases in the Yichang area showed high genetic diversity and low transmission rates.Clustering rates were not linked to whether cases were new or relapsed cases.Results suggest that the majority of tuberculosis cases in Yichang are due to endogenous activation.There is also a limited amount of local transmission.Greater attention should be paid to tuberculosis prevention and control in Yichang.
【Fund】: 湖北省卫生与计划生育委员会联合基金项目(WJ2018H241)
【CateGory Index】: R446.5;R521
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LIN Gui-wei;WANG Yong-lin;Xianju County Center for Disease Control and Prevention;;Analysis of the VNTR genotyping of 80 Mycobacterium tuberculosis clinical strains isolated from Xianju[J];中国卫生检验杂志;2015-06
2 XU Peng;GAN Ming-Yu;GAO Qian;Key Laboratory of Medical Molecular Virology of Ministries of Education and Health,School of Basic Medical Sciences,Fudan University;;Current applications of next-generation sequencing technology in Mycobacterium tuberculosis research[J];微生物与感染;2015-01
3 CHEN Zhi1,CHEN Jia2,LI Liu-lin1,XIAN De-qiang1,YUE Yun-yao1,JIANG Zhi-hui1,ZHOU Liang-jun1.1.Department of Physico-Chemical Laboratory,Mianyang Center for Disease Control and Prevention,Mianyang 621000,China;2.Department of Pediatrics,Central Hospital of Mianyang City,Mianyang 621000,China;Genotyping of mycobacterium tuberculosis with VNTR technique in Mianyang,Sichuan[J];中华疾病控制杂志;2011-09
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 LIU Xiao-jun;YU Feng-hua;YU Yun-fang;JI L-yu;ZHOU Pan;ZHAO Yan-lin;Clinical Laboratory, Yichang Center for Disease Control and Prevention,Hubei Province;;MIRU-VNTR genotyping and clustering of Mycobacterium tuberculosis isolates in Yichang[J];中国防痨杂志;2019-03
2 ZHANG Si-chao;DONG Zheng-quan;YANG Zhong-rong;The Center for Disease Control and Prevention of Huzhou City;;A study on genotyping of mycobacterium tuberculosis by variable number tandem repeat analysis method[J];浙江预防医学;2014-03
3 ZHU Xue-bin 1,LI Shi-ming 2,ZHANG Jin-ling 1,SONG Lei 1,ZHANG Zhong-rui 1,HE Lei 1,GAO Li 1,SHI Zhe 1,FENG Fu-min 1.1.School of Public Health,Hebei United University,Key Laboratory of Occupational Health and Safety,Tangshan 063000,China;2.Department of Clinical Laboratories,Tangshan Tuberculosis Hospital,Tangshan 063000,China;The genotype analysis of crowd Mycobacterium tuberculosis in Tangshan[J];中华疾病控制杂志;2013-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LEI Yong-liang,CHEN Xiu-ying,MEI Jian-hua,TAO Yi,LAN Jin-quan,WANG Xiao-guang (The Lishui Center for Disease Prevention and Control,Lishui 323000,China);Study on the genotyping of 87 Mycobacterium tuberculosis isolates in Lishui based on 15 variable number of tandern DNA repeats[J];中国卫生检验杂志;2012-09
2 DONG Hai-yan,YANG Jun,ZHAO Xiu-qin,LI Ding-yue,CHEN Jian,YANG Yun,LIU Zhi-guang,LONG Bo,WAN Kang-lin State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Genotyping of Mycobacterium tuberculosis strain isolated from Sichuan province by variable number tandem repeat analysis method[J];中国预防医学杂志;2012-01
3 ZHU Hai-yan,WU Fang,QIAN Xue-qi,et al(Haining Center for Disease Prevention and Control,Haining 314400,China);Genotyping of MLVA isolated from Mycobacterium tuberculosis clinical strains[J];中国农村卫生事业管理;2010-11
4 MI Li-gu~*,WANG Yuan-zhi,LI Yong-xiang,Arkenjiang·zipar,CAO Hong-yan,WU Chang-dong, CHEN Wei,YUAN Li.* Xinjiang Shi He-zi University School of Medicine Department of Pathogenic Biology and Immunology,Shi He-zi 832000,China;Genotyping analysis of 151 cfinical isolates of Mycobacterium tuberculosis from Uygur in south of Xinjiang[J];中华微生物学和免疫学杂志;2010-07
5 ZHENG Xiao-jing,ZHENG Su-hua,DU Bo-ping,JIA Hong-yan,LI Wei-min,DING Bei-chuan,WANG Su-min,ZHANG Zong-de.Laboratory of Molecular Biology for Mycobacteria,Beijing Tuberculosis and Thoracic Tumor Research Institute,Beijing 101149,China;The application of multiple loci variable number tandem repeats analysis in genotyping of Beijing family Mycobacterium tuberculosis strains[J];临床肺科杂志;2010-07
6 WANG Bo1,YUANG Yan-li1,GUO Jian-hua1,LIU Zhi-guang2,WANG Yan-hua1,YANG Xiu-jun1,WANG Kang-lin2(1.Jilin Center for Disease Control and Prevention,Changchun 130062,China;2.National Institute of Communicalbe Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China);Genotyping of Mycobacterium tuberculosis in Jilin Province with VNTR[J];中国卫生检验杂志;2009-06
7 LU Bing LI Zhao-na LIU Mei LIU Zhi-guang ZHAO Xiu-qin WAN Kang-lin State Key Laboratory for Infectious Diseases Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Discrimination power evaluation for 45 loci of variable number tandem repeats in Mycobacterium tuberculosis strains isolated from China[J];中华流行病学杂志;2009-01
8 LIU Zheng-wei~* LBing WANG Xiao-meng,et al.* Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China;Analysis on the Genotyping of 71 Mycobacterium Tuberculosis Clinical Strains Isolated from Zhejiang Province with MLVA[J];中国预防医学杂志;2008-12
9 WANG Xiao-meng1,LU Bing2,LIU Zheng-wei1,LIU Jie2,HE Hai-bo1,JIANG Yi2,DONG Hai-yan2,LIU Zhi-guang2,ZHAO Xiu-qin2,WAN Kang-lin2 (1.Center for Disease Control and Prevention Zhejiang Province;2.State Key Laboratory for Infectious Disease Prevention and Control;National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention);Genotyping of Mycobacterium tuberculosis clinical isolates in Zhejiang province with spacer oligonucleotide typing and multiple loci variable number tandem repeat analysis[J];中国人兽共患病学报;2008-12
10 LU Bing* Christine POURCEL LIU Jing-hua DONG Hai-yan ZHANG Yuan-yuan JIANG Yi LIU Zhi-guang ZHAO Xiu-qin WAN Kang-lin *State Key Laboratory for Infectious Diseases Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Establishment of the standard operation program on multiple loci variable numbers tandem repeats analysis typing on Mycobacterium tuberculosis[J];中华流行病学杂志;2008-09
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved