ESTABLISHING OF A NEW CISPLATIN-RESISTANT CELL LINE AND STUDING OF ITS MECHANISM CHARACTERIZATION OF DRUG-RESISTANCE
Huang Wei; Li Li; Tang Dongping; (Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021.)
To establish cis-platin (DDP)-resistant human ovarian cancer cell line and investigate it'S featureand mechanism reponsible of resistance of DDP. METHODS DDP-resistant human ovarian cancer line (A2780/DDP) was developed by gradually increasing dose and the sensitivity of the anticancer drugs were screened in theA2780/DDP by MTT method. DDP content in the A2780/DDP was screened by High-performance liquid chromatography (HPLC). The polymeras chain reaction (RT-PCR) and the immunohistochemical method were used tomeasure multidrug resistance (MDR1) and Glutathione transferase-π (GST-π). RESULTS (1) A2780/DDP cellswere of 10. 59-fold resistance DDP and displayed high resistance to DDP and displayed high resistance with VCR、MTX, but low resistance with Carp、Pym、CTX、 VP16. No cross-resistance was found to ADM, 5-FU and KSM.(2) DDP content of the A2780/DDP decreased significantly. (3) There was higher over expression of GST-π inA2780/DDP and lower were expression of MDR1 in A2780/DDP with high DDP resistance. CONCLUSIONA2780/DDP cells was acquired and had a characterization of the multidurg resistance. The primary factor of causing A2780/DDP resistance to DDP is correlate overexpression of GST-π and the reductive intracellular content ofDDP.
【CateGory Index】： R737.31