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《Chinese Journal of Grassland》 2010-05
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ISSR Analysis on Chromosome Doubling Plants F_2 of Reciprocal Hybrids between Agropyron mongolicum and A. cristatum cv. Fairway

BAO Mei-lian,YU Zhuo,MA Yan-hong,SU He,LI Xiao-yu(Agronomy College,Inner Mongolia Agricultural University,Hohhot 010019,China)  
To determine the degree of genetic difference among chromosome doubling plants F2 of reciprocal hybrids F1 between Agropyron mongolicum and A.cristatum cv.Fairway,their parents and reciprocal hybrids F1,and provide basis for selection of good lines from tetraploid chromosome doubling progenies,the polymorphism among the tested plants were analyzed based on ISSR molecular marker.The results showed that 515 bands of ISSR markers were amplified with 12 suitable primers,492 bands were polymorphic,and the percentage of polymorphism bands reached 95.3%.The genetic distance(GD) of 6 tested plants were ranged from 0.4094 to 0.6721,and the average of genetic distance was 0.5391.The genetic distance between A.cristatum cv.Fairway and A.mongolicum,between A.cristatum cv.Fairway and chromosome doubling plants F2 of reciprocal hybrids was wide,and then the genetic distance between Agropyron mongolicum × A.cristatum cv.Fairway F1 and A.cristatum cv.Fairway ×A.mongolicum F1,and the genetic distance among chromosome doubling plants F2 of reciprocal hybrid was the nearest.Using the GD value of 0.51 as threshold,6 tested plants could be clustered into 3 groups: the first group including A.mongolicum and inverse cross F1,the second groups including obverse cross F1 and chromosome doubling plants F2 of reciprocal hybrids,and the third groups including A.cristatum cv.Fairway.
【Fund】: 国家科技支撑计划项目“牧草育种技术研究及产业化开发——牧草倍性育种技术研究”(2008BADB3B03);; 国家重点基础研究发展计划(“973”计划)项目“中国西部牧草、乡土草遗传及选育的基础研究”(2007CB108901);; 教育部博士点基金项目(20091515110002);; 内蒙古自然科学基金项目(2009M50506)
【CateGory Index】: S543.9
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