The Analysis on Peroxidase (POD) Isozyme of Several Wide Species and Their Hybrids in the Triticeae.
YU Zhuo (Department of Desert Control, Inner Mongolia Forestry College, Hohhot 010019), YUN Jin feng (Department of Grassland Science, Inner Mongolia Institute of Agriculture and Animal Husbandry, Hohhot 010018)
The POD isozyme of Elymus canadensis, Hordeum brevisbulatum and their F 1 hybrid, Agropyron mongolicum, Agropyron cristatum and their F 1 hybrid and backcross 1 in Triticeae were analyzed by polyacrylamide gel electrophoresis. The results showed: the POD zymograms of each species can be divided into two regions (A and B). There were two isozyme bands at same location, which were parental base bands. All experiment materials had 6～9 isozyme bands. There were differences in the zymograms between parents and their hybrids. There was polymorphism in the POD isozyme and it can be used as genetic marker to identify hybrid and to determine the plants with object characteristics in backcross descendants. The E. canadensis-H. brevisubulatum F 1 hybrid had parental isozyme bands with higher activity. It probably possesses of some superior characteristics of parents.