Studies on Dot Immunogold Filtration Assay for Detection of Antibody to Classical Swine Fever Virus
Kong FanDe Huang YinYao Lai Qingjin Hong YiZhun Lu ChengPing (1 Nanjing Agricultural University,Nanjing 210095; 2 Xiamen Exit - Entry Inspection and Quarantine Service of P.R. of China,Xiamen 361012; 3 Zhangzhou Animl Husbandry and Veterinary Station)
To establish a more rapid and simple method of detecting antibody of SFV, a dot immunogold filtration assay (DIGFA) was developed by coating the antigen of CSFV on nitrocellulose (NC) membrane, and labeling staphylococcal protein A (SPA) with colloidal gold.The color of the positive dots were so bright that the results could be easily determined.The whole procedure could be finished in 5 min.The method had a better specificity without cross - reaction with positive sera to PKRSV, PRV, PPV, NDV and AFV.Its coincidence with Dot - EUSA,indirect haemagglutination test(IH) were 98.4% and 98.9% respectively.DIGFA,more simple and rapid,was applicable to small diagnostic laboratories.lt could be applied for more early diagnosis ^ epidemiological investigation and the detection of antibody level in vaccinated herds.
【CateGory Index】： S852.4