Diagnosis of the First Outbreak of Peste des Petits Ruminants in Tibet
The first outbreak of peste des petits ruminants in ovine in Tibet was confirmed by multiple laboratory tests including one step real-time RT-PCR, conventional RT-PCR, competitive ELISA and virus isolation. The presence of PPRV was detected in 11 out of 13 field samples by one step real-time RT-PCR, among which 8 were further detected by conventional RT-PCR. Sequence analysis showed that the virus fell into the lineage 4 which was epidemic in Middle East and certain western Asian countries. One strain of PPRV was isolated in Vero cell lines from field samples and confirmed by RT-PCR test.
【CateGory Index】： S858.27