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《Chinese Journal of Comparative Medicine》 2010-03
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Comparison of Two Method about Antibody against Herpesvirus Saimiri from Rhesus Macaques

QIAO Hong-wei,TONG Wei,JIANG Hong,CONG Zhe,WANG Wei,WEI Qiang(Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences and Peking Union Medical College;Comparative Medical Center,Peking Union Medical College;Key Laboratory of Human DiseaseComparative Medicine,Ministry of Health,Beijing 100021,China)  
Objective To compare the serological detection sensitivity of sera against HSV-1 and BV from humans and rhesus monkeys with PR(plaque reduction)and IFA(immunofluorescence assay).Methods The PR assay described in this study was modified from the traditional viral plaque reduction assay by using with 1 mL of DMEM medium containing 1%(weight/volume) of methylcellulose(4000 cP;Sigma,MC),and an improved crystal staining method to achieve better plague formation in HSV-1 infected Vero E6 cells.The neutralizing antibody titer was determined as the highest serum dilution which achieved 50% plaque reduction.Results In this assay,HSV-1 infected Vero E6 cells formed clear plaques with the density of(115~116) dots/mL.The positive serum neutralizing antibodies titers and ratios were 1∶80 and 100% both of HSV-1 and BV.Conclusions The PR assay described in this study will be used not only for serological detection but also for medical selection in cell model.
【Fund】: 中央级公益性科研院所级本科研业务费项目 项目号:DWS200708;; 科技部基础专项重点项目 项目号:2002DEA20023;; 科技部条件基础平台专项重点项目 项目号:2003DIA7J033
【CateGory Index】: R446.6
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 WEI Yi,FU Min-tai,SHI Han The Research Center of primate laboratory Animals of Guangxi TIAN Ke\|gong,YU Xiu\|ling Laboratory Animal Center in Academy of Military Medical Sciences;A Comparison Study on the Result of Monkey's B Virus by Using Three Different Antigens[J];Laboratory Animal Science and Administration;2001-02
2 Wu Xiao-Xian et al(Institute of Medical Laboratory Animal, Chinese Academy of Medical Sciences, Beijing);Study on the Monitoring Methods for B Virus Related Antibody in Rhesus Monkey[J];Virologica Sinica;1989-01
3 RAO Jun-hua1, LIU Xiao-ming1, TIAN Ke-gong2, YU Xiu-ling3, JIN Shi-jun1, WANG Hai-ying1 (1 Primate Research and Development Center of South-China Institute of Endangered Animals, Guangzhou 510555,China;=2.National Veterinary Diagnostic Center of Ministry of Agricuture;=3.Laboratory Animal Center of Academy of Military Medical Science);Study on BV Antibody Surveillance in Establishment and Maintenance of Macaca mulatta SPF Colony[J];Chinese Journal of Laboratory Animal Science;2003-05
4 TIAN Kegong, LIN Huiyun, YU Xiuling, LI Jingyun, BAO Zuoyi, WU Na (Laboratory Animal Center, Academy of Military Medical Science, Beijing 100071,China);Identification of Simian B Virus by Polymerase Chain Reaction[J];Acta Laboratorium Animalis Scientia Sinica;2001-04
5 Lu Yaozeng, et al( Institute of Laboratory Animal Science, CAMS, Beijing);The Establishment of SIV-Infected MacaqueModels[J];ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA;1994-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Shen YongZhou et al (Anhui Agriculture University,Hefei 230036);Serological Survey of Canine Reovirus Type 3[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1999-01
2 SUN Rui (Zhengzhou Railway Vocational and Technical College,Zhengzhou,Henan 450052);Comparative Study of Histopathology on Canine Distemper and Canine Parvovirus Disease[J];Journal of Anhui Agricultural Sciences;2008-13
3 ZHANG Gao-hong, LI Ming-hua, ZHENG Yong-tang (Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Diseases Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, China);Application of AIDS Macaque Animal Model in HIV Vaccine Research[J];Zoological Research;2007-05
4 SHI Chang hong,LI Liu jin,LI Cheng,LI Hong (The labarotory Animal Research Center,Fourth Millitary Medical University,Xian,710032,China);Observation on the Poxvirus Infection in KM and BALB/c Mice[J];Progress In Veterinary Medicine;2002-03
5 MIN Fan-gui,WU Yu-e,LI Wen-de,PAN Jin-chun,LIU Zhong-hua,HUANG Ren(Guangdong Laboratory Animals Monitoring Institute,Guangzhou,Guangdong,510260,China);Analysis of Immunodeficiency in an Old Macaca fascicularis[J];Progress in Veterinary Medicine;2006-09
6 Wei Yi; Liu Zimin; Huang Guangbin; Fu Mingtai(Guangxi Research Centre for Experimental Primates,133 Qixing Road,Nanning,Guangxi,530022)Liu Feng(The Health Epidemic Prevention Station of He County,542800);A Preliminary Research on B Virus Related Antibody of Macaca fascicularis[J];GUANGXI SCIENCES;1996-01
7 Wei Yi;Liu Zimin;Huang Guangbin;Mai Zaoliang;Fu Mingtai;Liu Feng(Guangxi Research Center for ExperimontalPrimate,133 Qixing Road,Nanning, Guangxi, 530022);An Exploration of Measles Virus Infected State in Macaca mulatta from Longhu Mountains[J];GUANGXI SCIENCES;1996-02
8 Ma Xiuzhen Hu Gang (Nanning Animal Quarantine Service of P R China, (Southwest China Forestry 51 North Youailu,Nanning,Guangxi,530001) College,Kunming,Yunnan,650224) Tang Wenhong Weng Jian (Dept. of Biology,Guangxi Normal U;On Detecting B Virus Related Antibody in Rhesus Monkey from Guangxi[J];GUANGXI SCIENCES;1997-02
9 Tuang Wenhong 1 ) Wei Yi 2 ) Hu Gang 3 ) ( 1)Department of Biology, Guangxi Normal University,541004,Guilin,PRC; 2)Station of Guangxi Wildlife Conservation,530022, Nanning,PRC; 3)Department of Forestry protection, South west Forestry Couege,;QRARANTINE STUDY OF B VIRUS OF M. ARCTOIDES[J];JOURNAL OF GUANGXI NORMAL UNIVERSITY(NATURAL SCIENCE);1997-01
10 Li Ping (李 平), Guan Chongfen (关崇芬) (The Institute of Basic Theory, China Academy of Traditional Chinese Medicine, Beijing 100700);An Initial Study on Mechanism of Zhong Yan Ⅱ in Antiviral Activity Against Simian Immunodeficiency Virus[J];JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE;2000-01
China Proceedings of conference Full-text Database 1 Hits
1 HE Zhengming WEI Li GONG Wei TIAN Kegong JIA Ruisheng (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050) (1. National Veterinary Diagnostic Centerl, Ministry of Agriculture, Beijing, 100094) (2. Institute of Laboratory Animal, Chinese Academy of Medical Science, Beijing 100021);Establishment and Application of ELISA for Detection of Antibody to Simian B Virus[A];[C];2002
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Wei Yi; Liu Zimin; Huang Guangbin; Fu Mingtai(Guangxi Research Centre for Experimental Primates,133 Qixing Road,Nanning,Guangxi,530022)Liu Feng(The Health Epidemic Prevention Station of He County,542800);A Preliminary Research on B Virus Related Antibody of Macaca fascicularis[J];GUANGXI SCIENCES;1996-01
2 Ma Xiuzhen Hu Gang (Nanning Animal Quarantine Service of P R China, (Southwest China Forestry 51 North Youailu,Nanning,Guangxi,530001) College,Kunming,Yunnan,650224) Tang Wenhong Weng Jian (Dept. of Biology,Guangxi Normal U;On Detecting B Virus Related Antibody in Rhesus Monkey from Guangxi[J];GUANGXI SCIENCES;1997-02
3 Zhou Yusen, He Zhongping, Dong Jingfang, Wang Haitao Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071;Cloning and sequencing of partial gene from TTV isolate in China[J];BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES;1998-02
4 RAO Jun-hua *, LIU Xiao-ming, TIAN Ke-gong, YU Xiu-ling, WANG Hai-ying ( *Primate Research & Development Center of South-China Institute of Endangered Animals, Guangzhou, 510555, China);Comparative Study on BV EIA and BV ELISA in B Virus Antibody Detection in Cynomolgus Monkey[J];Laboratory Animal Science and Administration;2001-04
5 Wu Xiao-Xian et al(Institute of Medical Laboratory Animal, Chinese Academy of Medical Sciences, Beijing);Study on the Monitoring Methods for B Virus Related Antibody in Rhesus Monkey[J];Virologica Sinica;1989-01
6 Wu Xiaoxian, et al ( Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences );Study on Virologic Monitoring of Rhesus Monkey[J];;1991-02
7 Lu Yaozeng, et al( Institute of Laboratory Animal Science, CAMS, Beijing);The Establishment of SIV-Infected MacaqueModels[J];ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA;1994-02
8 Jiang Guancheng, et al (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Bejinl00021);Detection of Enteropathogens and Observation of the Pathogenesis in Monkeys[J];Chinese Journal of Zoonoses;1993-04
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