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《Chinese Journal of Comparative Medicine》 2010-07
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RAPD Analysis of the Genetic Structure for Closed Colony of Brandt's Vole

SHI Hai-xia,PAN Si-dan,LIANG Hong,SONG Ming-jing (Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS) & Comparative Medicine Centre,Peking Union Medical College (PUMC),Beijing 100021,China)  
Objective To establish the he genetic structure for cultivation population of Brandt's vole (Lasiopodomys brandtii) by RAPD (randomly amplified polymorphic DNA) assay. Method Genomic DNA was extracted from tail tip following high-concentration-salt precipitation methods. 40 RAPD primers were PCR amplified to analyze the DNA structure of Brandt's vole population. RAPD markers were separated by agarose gel electrophoresis. Alleles were determined according to a size marker. Monomorphic site,polymorphic site and the proportion of polymorphic loci,were used to describe the genetic variation of Brandt's vole population detected by RAPD. Results The rates of variation of RAPD loci by 8 primers used in this study are different. The combination of the PCR bandings by eight RAPD primers can provide sufficient statistical power to assess the genetic variations in Brandt's vole population. Conclusion We have successfully established genetic methods for analyzing genetic structure of closed colony of Brandt's vole.
【Fund】: 高等学校博士学科点专项科研基金(20070023119);; 中央级公益性科研院所基本科研业务费专项基金(DWS200702);; 国家科技支撑计划课题(2009BAI83B01)子课题资助
【CateGory Index】: R-332
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