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《Chinese Journal of Comparative Medicine》 2010-07
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RAPD Analysis of the Genetic Structure for Closed Colony of Brandt's Vole

SHI Hai-xia,PAN Si-dan,LIANG Hong,SONG Ming-jing (Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS) & Comparative Medicine Centre,Peking Union Medical College (PUMC),Beijing 100021,China)  
Objective To establish the he genetic structure for cultivation population of Brandt's vole (Lasiopodomys brandtii) by RAPD (randomly amplified polymorphic DNA) assay. Method Genomic DNA was extracted from tail tip following high-concentration-salt precipitation methods. 40 RAPD primers were PCR amplified to analyze the DNA structure of Brandt's vole population. RAPD markers were separated by agarose gel electrophoresis. Alleles were determined according to a size marker. Monomorphic site,polymorphic site and the proportion of polymorphic loci,were used to describe the genetic variation of Brandt's vole population detected by RAPD. Results The rates of variation of RAPD loci by 8 primers used in this study are different. The combination of the PCR bandings by eight RAPD primers can provide sufficient statistical power to assess the genetic variations in Brandt's vole population. Conclusion We have successfully established genetic methods for analyzing genetic structure of closed colony of Brandt's vole.
【Fund】: 高等学校博士学科点专项科研基金(20070023119);; 中央级公益性科研院所基本科研业务费专项基金(DWS200702);; 国家科技支撑计划课题(2009BAI83B01)子课题资助
【CateGory Index】: R-332
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1 SONG Guo-hua, LIU Tian-fu (Laboratory Animal Center, Shanxi Medical University, Taiyuan 030001, China);Study on RAPD Analysis System in Chinese Hamster[J];Chinese Journal of Laboratory Animal Science;2005-02
2 WANG Hong~1,YUE Bing-fei~1,WANG Ju~2,LIU Shuang-huan~1,HE Zheng-ming~1, XING Rui-chang~1(1.National Institute for the Quality Control of Pharmaceutical and Biological Products,Beijing 100050,China;2.Department of Laboratory Animals,Capital Medical University, Beijing);Comparison of RAPD Maps of Gerbil (Meriones unguiculatus) and Laboratory Rats and Mice[J];Chinese Journal of Comparative Medicine;2006-06
3 CAI Yue-qin,TU Jue,YU Jia,XIAO Hui,PAN Yong-ming,LU Ting,CHEN Min-li(Zhejiang University of Traditional Chinese Medicine Laboratory Animal Research Center,Hangzhou 310053,China);RAPD Analysis Used in Monitoring the Genetic Profile of WHBE Rabbit in Inbred Cultivation[J];Acta Laboratorium Animalis Scientia Sinica;2009-05
4 LIANG Hong,PAN Si-dan,SHI Hai-xia,GAO Shan,ZHANG Jian-jun,SONG Ming-jing*(Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health;Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS) & Comparative Medicine Centre,Peking Union Medical College(PUMC),Beijing 100021,China);Establishment of cleaning grade closed colony of Microtus brandti and reproductive traits analysis[J];Chinese Journal of Health Laboratory Technology;2009-12
Chinese Journal Full-text Database 6 Hits
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3 HU Pei-li,FAN Chang-fa,YUE Bing-fei,XING Rui-chang(National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China);Monitoring the SNP Loci of 4 Inbred Mice[J];Chinese Journal of Comparative Medicine;2006-05
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6 PAN Si-dan,SHI Hai-xia,LIANG Hong,SONG Ming-jing(Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS) & Comparative Medicine Centre,Peking Union Medical College(PUMC),Beijing 100021,China);Development of experiment animalization breeding technique and analysis of organ coefficient for Lasiopodomys brandtii[J];Chinese Journal of Health Laboratory Technology;2010-05
China Proceedings of conference Full-text Database 1 Hits
1 WANG Hong YUE Bing-fei LIU Shuang-huan HE Zheng-ming XING Rui-chang (National Institute for the Quality Control of Pharmaceutical and Biological Products, Beijing 100050);Comparing RAPD map and STR map with the map of biochemistry method in monitoring inbred mouse BABL/c[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wu Fengchun, Wei Hong (Laboratory Animal Center, Third Military Medical University, Chongqing, 400038);Optimizing of RAPD PCR procedure in mice[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;1999-01
2 QIN Chuan~(1,2),DENG Wei~(2),ZHANG Lian-feng~(2),CONG Bin~(1*) (1.Hebei Medical University,Shijiazhuang 050071;2.Institute of Laboratory Animal Science,CAMS & PUMC,Bejing 100021,China);Pathological changes on SARS-CoV infected Microtus brandtii, Lewis rat and Rhesus macaque[J];Basic & Clinical Medicine;2006-03
3 LIU Li jun, TANG Yi ming, FU Jie, XIE En,SHI En,XU Ping(Shanghai Laboratory Animal Center, Academy Science of China, Shanghai 201615, China);Development of Early-stage Embryos of Microtus fortis,in vivo and in vitro[J];Journal of Shanghai Jiaotong University (Agricultural Science);2003-S1
4 LIU Li-jun1,LU Ye2,TANG Yi-ming1,SHI En 1,XU Ping 1(1.Shanghai laboratory animal center,Academy science of China,Shanghai 2016 15;2.School of Life Science ,Suzhou University,Suzhou 215007,China);The in Vitro Fertilization by Microtus Fortis Spermotoas in Fresh and in Post Frozen-Thawed[J];Journal of Shanghai Jiaotong University (Agricultural Science);2004-03
5 ZHAO Li-hu1,2, XIE Xia-yang1, ZHAO Dan-feng1,2, ZHANG Chun-yan1,3, DI Min1,4 LIU Li-jun1, Li Sheng-yang2, ZHANG Shou-chun2, XU Ping1 (1. Shanghai Laboratory Animal Center, Academia Sinica, Shanghai 201615,China; 2.College of Animal Science and Veterinary, Shenyang Agricultural University, Shenyang 110161,China; 3.College of Veterinary, Nanjing Agricultural University, Nanjing 210095,China; 4. Comparative Medicine Center of Yangzhou University, Yangzhou 225009,Chin);RAPD Analysis of the Genetic Specificity in Laboratory Rabbits[J];Journal of Shanghai Jiaotong University(Agricultural Science);2007-01
6 Shen Zhi-ming; Yu Jia-huang(Shanghai Laboratory Animal Research Center, Shanghai 200032);Study on Domesticating and Breeding of Microtus fortis B chner[J];XHANGHAI LABORATORY ANIMAL SCIENCE;1995-02
7 SHEN Cheng-wen 1, LUO Jun-wu 1, LIU Chun-lin 2, TANG He-ping 1, HUANG Yi-huan 1 (1.College of Food Science and Technology, Hunan Agriculture University, Changsha 410128,Hunan,China; 2.Crop Gene Engineering Key Laboratory of Hunan Province, Hunan A;Study on RAPD Analysis System in Tea Plants[J];Life Science Research;2002-02
8 Zhang Wen-yan Gong Chun-mei Wei Yun-lin Wu Chun-hua Wang Wen Lan Hong Li Shang-ru Su Bin Zhang Ya-ping (Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, 650107) Cell and Molecular Evolution Laboratory, Kunming Institute of Zoology, the Chinese Academy of Sciences. Laboratory Animal Center of Beijing Institute of Academy of Military Medical Sciences.;A NEW WAY FOR INBRED STRAIN MICE GENETIC MONITORING AND THE DISCOVERY OF SEX-LINKAGING RAPD MARKERS[J];Acta Biologiae Experimentalis Sinica;1996-01
9 CHEN Min-li,CAI Yue-qin,TAO Tao,YU Jia,XIAO Hui,ZHU Liang,XU Xiao-ping,WANG De-jun,YING Hua-zhong(Zhejiang Chinese Medical University Laboratory Animal Research Center,Hangzhou 310053,China);Genetic Analysis of WHBE Rabbit,Japanese Rabbit and New Zealand Rabbit by Microsatellite Loci[J];Chinese Journal of Comparative Medicine;2008-09
10 HUANG Zhaofeng,ZHONG Naiqing,TAN Pingping,DUAN Ziyuan *,CHEN Weiping,LIU Yunzhong,LUO Tianyi (1.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Bejing 100101,China; 2.Guangzhou Pharmaccurical Industrial Research Institute;Analysis for Genetic Background of Laboratory Beagle Dog with Random Amplified Polymorphic DNA(RAPD)[J];Acta Laboratorium Animalis Scientia Sinica;2002-02
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