Expression of group A rotavirus VP7 gene in recombined Lactobacillus

WU Jing-hua,WANG Hai-xin,ZHAO Jie,et al.Clinical Laboratory,North China Coal Medical University(Tangshan 063000,China)  

Objective To construct vector pEDM27/5-VP7 by RT-PCR and transform it into Lacotobacillus for the construction of genetic engineering vaccine against rotavirus.Methods The VP7 gene fragment was isolated and recoveried by RT-PCR,and transformed into pEDM27/5.The gene was transformed into Lactobacillus through extraction from the plasmid.After lactose induction,the vp7 was detected with SDS-PAGE and Western-blot at 4,8 and 12 hours.Results The VP7 gene was transformed into pEDM27/5 successfully,and the susceptibity bacteria was recombined by pEDM27/5-vp7.After lactose induction at 4,8,12 hours,the protein(28kD) was detected with SDS-PAGE and Western-blot.The expressed protein was 2.30%,5.12%,5.38% of total protein of the bacteria,respectively by scanning,and the expression increased with the increment of the time.The protein could combined it specially combined with anti-vp7 protein.Conclusion The recombinant Lactobacillus expresses immunocompetent vp7 protein constantly,and could be used for further development of genetic engineering vaccine against rotavirus.

【Fund】： 唐山科技局课题(08130227C)
【CateGory Index】： R346

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