Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Public Health》 2013-09
Add to Favorite Get Latest Update

Genotyping of platelet antigens HPA-2 with melting curve analysis

CHEN Yu-ning;WANG Yan-wei;Department of Laboratory,The G eneral Hospital of Shenyang Military Region;  
Objective To develop a genotyping method for human platelet antigens-2(HPA-2) by using melting curve analysis and to investigate the gene frequencies of HPA-2 in Han population in Dalian city. Methods Real-time PCR was used and melting curve analysis was performed for genotyping of HPA-2. Reproducibility of the method was evaluated and the results of melting curve analysis were compared with those using traditional PCR amplification with sequence-specific primers(PCR-SSP) method. The genotype of HPA-2 in 155 Han platelet donors were determined using melting curve analysis,then the gene frequencies of HPA-2 were compared with that of other populations in China Results Melting curve of HPA-2aa sample showed a single peak with the melting temperature(Tm) of 85 ± 1 ℃; melting curve of HPA-2ab sample showed two peaks with the Tm of 80 ± 1 ℃ and 85 ± 1 ℃; and melting curve of HPA-2bb sample showed a single peak,with the Tm of 80 ± 1 ℃. There was complete concordance of results for all samples tested by PCR-SSP and melting curve analysis. The gene frequencies of HPA-2a and HPA-2b in Han populations of Dalian city were 0. 9161 and 0. 0838,respectively. The distribution of HPA-2 alleles in Liaoning Han populations is similar to that in Nanjing,Shanghai,and Xinjiang Han and Xingjiang Uyghur populations(P 0. 05 for all),but it significantly differ from that in Hainan Li national minority people(P 0. 05). Conclusion The method for genotyping of HPA-2 by using melting curve analysis was developed and the method is faster,simpler,and more accurate compared to the results of traditional PCR-SSP. There are differences in HPA-2 genotypes among different race in China
【CateGory Index】: R450
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 ZHOU Wei1 WANG Hanping2 XIE Jianjin1 YING Yi1 CHEN Xiaowei1 CHEN Xiaoyan1(1Department of Hematology,First Municipal People's Hospital of Guangzhou,Guangzhou,510180,China;2 Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics,First Municipal People's Hospital of Guangzhou);Retrospective study on high resolution melting curve analysis for detecting JAK2V617Fmutation in 112patients[J];Journal of Clinical Hematology;2012-04
2 MAO Hai-yan,LU Yi-yu,CHEN Yin,et al.Institute of Virology,Zhejiang Provincial Center for Disease Control and Prevention(Hangzhou 310051,China);Development of SYBR Green real-time PCR assay for detection of adenovirus[J];Chinese Journal of Public Health;2010-01
3 LI Ru,LIAO Can,LI Dong-zhi,LI Jian.(Institute of Healthy birth and Perinatology,Guangzhou Women and Children's Medical Center,Guangzhou,510623);High-resolution melting analysis of common HBB gene mutations in β-thalassemia.[J];Chinese Journal of Birth Health & Heredity;2011-07
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHEN Weidong;LI Lilan;LIAO Yan;ZHAO Tongmao;WU Guoguang;Nanning Institute of Transfusion Medicine;National Institutes of Health,NIH,USA;;Study on multi-PCR-SSP technique for HPA-1 ~ 17bw genotyping and the distribution of HPAs polymorphism in Chinese Zhuang population[J];Chinese Journal of Blood Transfusion;2014-01
2 ZHU Xiang-ming,YANG Tong-han,YAO Fu-zhu,et al.Kunming Blood Center of Yunnan Province,Kunming 650106;Gene Frequencies of the HLA-A,B and HPA in Typed Platelet Donors of Yunnan Han Nationality[J];Journal of Clinical Transfusion and Laboratory Medicine;2012-03
3 MIAO Jing;ZENG Xiao-jing;Department of Hematology,the Affiliated Hospital of Guiyang Medical College;;Correlation between polymorphism of HPA-1~6,15 and the effect of platelet transfusion in patients with acute leukemia[J];Hainan Medical Journal;2014-03
4 XIA Lan,CAI Xingquan,FU Xiao-ling(Hainan Provincial People's Hospital,Haikou 570311,Hainan,P.R.China);Significance of gentyping of platelet antigen in random infusion of paletlet[J];China Tropical Medicine;2009-06
5 DENG Jian-qiang;LI Wen-hui;Li Liang;Department of Forensic Medicine,Hainan Medical College;;Preliminary study of species identification by HRM in forensic entomology[J];China Tropical Medicine;2014-01
6 YANG Bo;LV Yunlai;ZHU Lili;LV Sumei;CHEN Shanhua;Blood Center of Luoyang;;Investigation of frequency distribution of human platelet antigen-1to-10 in Luoyang[J];Journal of Clinical Hematology;2014-03
7 ZHU Xiang-ming,YANG Tong-han,YAO Fu-zhu,et al.(Blood Center of Kunming,Yunnan 650106,China);Analysis of gene polymorphism of human platelet antigen 1-17 system in platelet donors of Kunming[J];Medicine and Pharmacy of Yunnan;2011-05
8 GUO Jin-jin,SUN Xiao-hong,YAN Qing-li,et al.Chengde Medical College(Chengde 067000,China);Establishment of TaqMan real-time PT-PCR for detection of flavivirus[J];Chinese Journal of Public Health;2012-04
9 WANG Jian-guang,LEI Zhi-wen,LIU Yun-guo,et al.Department of Microorganism Detection Technical Center,Shandong Entry-Exit Inspection and Quarantine Bureau(Qingdao 266042,China);Detection of Shigella with helicase-dependent isothermal DNA amplification technology[J];Chinese Journal of Public Health;2012-04
10 HAO Bao-lan,WANG Yan*,SHAO Shu-jun,LIN Chang-qing,DONG Xiao-feng(Henan Tumor Hospital,Zhengzhou 450008,China);Research on platelet crossmatch by MACE in platelet transfusion of tumor patients[J];Chinese Journal of Health Laboratory Technology;2012-12
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 XIE Jian-jin,ZHOU wei,WANG Han-ping,et al.(Guangzhou Municipal First People's Hospital,Guangzhou 510180,Guangdong,P.R.China);Detection of the JAK2 V617F mutation in chronic myeloproliferative disorders by high resolution melting curve analysis[J];China Tropical Medicine;2011-04
2 LUO Yu,BIAN Guang-lin.Henan Institute of Medical Science(Zhengzhou 450052,China);Detection bacteria fragile by SYBR Green real-time PCR[J];Chinese Journal of Public Health;2007-01
3 ZHANG Tong, XU Chengbin, WANG Shenwu. Department of Cardiology, People′s Hospital, Beijing Medical University , Beijing 100044;Correlation between the polymorphism of platelet glycoprotein Ib gene and myocardial infarction[J];CHINESE JOURNAL OF CARDIOLOGY;1999-06
4 CHEN Fangping, JIAN Zaifu, XIE Qinzhi, et al. Department of Hematology, Xiang Ya Hospital, Hunan Medical University, Changsha 410008, China;Polymorphism of human platelets alloantigens in Chinese patients with acute myocardial infarction and acute ischemic stroke[J];NATIONAL MEDICAL JOURNAL OF CHINA;2000-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved