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《Chinese Journal of Respiratory and Critical Care Medicine》 2010-03
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Effects of Adiponectin on Proliferation of Airway Smooth Muscle Cells and Activation of Adenosine Monophosphate-Activated Protein Kinase

GUO Mao-qing,ZHU Shu-yang.Department of Respiratory Medcine,Affliated Hospital of Xuzhou Medical College.Xuzhou,Jiangsu,221002,China  
Objective To investigate the effect of adiponectin on proliferation of airway smooth muscle cells(ASMCs),and explore its possible mechanism.Methods ASMCs were derived from rat airway tissue and were cultured in vitro.RT-PCR was used to verify the expression of adiponectin receptors on ASMCs.Then ASMCs were treated with adiponectin at different concentrations(5,10,20,40,80 μg/mL) for different periods of time(1,12,24,48,72 hours),respectively.The Absorbsence ratios of adiponectin at different concentrations were determined by MTT assay.The adenosine monophosphate-activated protein kinase(AMPK) and phosphorylated AMPK(pho-AMPK) in ASMCs were quantified by Western blot after being treated with adiponectin at different concentrations(5,10,20,40 μg/mL) for 48 hours.Results The inhibition of adiponectin on ASMCs was showed in dose-dependent manner(r=0.324,P0.01) and time-dependent manner(r=0.607,P0.05).Western blot indicated that the expression of pho-AMPK increased with the increased concentrations of adiponectin(r=0.607,P0.01).The ratio of pho-AMPK/AMPK were(27.66±1.03)%,(31.91±0.86)%,(75.52±2.67)%,and (84.50±1.05)%,respectively,with significant differences between each concentrations of adiponectin(P0.05).There was no expression of pho-AMPK in the control group.Conclusion Adiponectin can significantly inhibit ASMCs’ proliferation by activating AMPK.
【CateGory Index】: R562.25
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