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《Chinese Journal of Respiratory and Critical Care Medicine》 2014-02
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A549 Cells Promote HUVEC Migration and Angiogenesis under Hypoxic Conditions

Hu Zan;Li Min;Hu Chengping;Mo Guiyan;Gu Qihua;Department of Respiratory Medicine,Xiangya Hospital,Central South University;  
Objective To observe the effects of A549 cells under hypoxicconditions on the migration of human umbilical vein endothelial cells(HUVECs) and microvascular formation.Methods After cultured for 24 h in normoxia condition(21% O2),hypoxia condition(2% O2),and anaerobic condition(0% O2),respectively,morphology of A549 cells was observed with inverted phase contrast microscope,proliferation was detected by MTT assay,and intracellular hypoxia-inducible factor-1α(HIF-1α) protein was detected by immunocyto-chemical technique,for determining whether the hypoxia model is successful.Then A549 cells' supernatant in the normoxic group,the hypoxia group and HUVECs culture medium were taken to intervene HUVECs.The migration of HUVECs was observed with cell scratch test,pseudopodia formation of HUVECs was observed with microfilament green fluorescent staining method,and blood vessel formation was observed with three-dimensional culture techniques in vitro.Results Compared with the normoxic group,the growth of A549 cells was better in the hypoxia group with more proliferation,and was poor in the anaerobic group with decreased number of cells.A549 cells in the hypoxia group and the anaerobic group both expressed HIF-1α protein,which was more obvious in the anaerobic group.Compared with the HUVECs supernatant intervention group,the hypoxia supernatant intervention group and the normoxic supernatant intervention group both had varying degrees of migration,pseudopodia structure formation and vascular lumen sample structure formation,which were more obvious in the former group.Conclusions A549 cells in hypoxic environment grow very well,proliferated significantly,but anaerobic environment is not conducive to the growth of A549 cells which found to be apoptosis.A549 cells in hypoxic environment can promote HUVECs migration,pseudopodia formation and angiogenesis.
【Fund】: 十二五国家科技支撑计划项目(编号:2013BAI09B09)
【CateGory Index】: R730.2
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