The study of erythropoiesis inhibitors in patients with uremia
SUN Qian-mei, WANG Zhi-gang. Department of Nephrology, ChaoyYang Hospital Affiliated to the Capital University of Medical Sciences, Beijing 100020, China
Objective To investigate the existence of the erythropoiesis inhibitors(EI) and the different effects of high-flux dialyzer and low-flux dialyzer on the clearance of EI. And to observe the effects of uremic patients sera and the ultrafiltrate from F80 and F6 dialyzer on CFU-E and BFU-E colony groewth of normal mice bone marrow. Methods The present study included twelve pre-dialysis ESRD patients with anemia.The sera from the uremic patients were added to CFU-E and BFU-E culture of normal mice bone marrow in the concentrations ranging from 1.25% to 5%.In vitro CFU-E and BFU-E growth in the presence of sera from ESRD patients was compared with those in the presence of seva from normal human subjects. The ultrafiltrate by F80 dialyzers was defined as group A. The ultrafiltrate of group A was recirculated and filtrated by F6 dialyzers,the remained ultrafiltrate was defined as group B, the ultrfiltrate from F6 dialyzers was as group C. The ultrafiltrate of the three groups were added to CFU-E and BFU-E culture in the concentrations ranging from 1.25% to 5%. In vitro CFU-E and BFU-E growth in the presence of ultrafiltrate of above three groups was compared with the CFU-E and BFU-E growth cultured with normal control’s sera. Results There was no significant inhibitory effect on the CFU-E and BFU-E growth of mice bone marrow cultured in vitro with the sera of normal subjects. The inhibitory effects of ultrafiltrate in different concentrations ranging from 1.25% to 5% on CFU-E and BFU-e colony growth were dose dependent. The ultrafiltrate of the ESRD patients had inhibitory effect on in vitro CFU-E and BFU-E growth of mice bone marrow.At the same ultrafiltrate of group A had more severe inhibitory effects on the growth of CFU-E and BFU-E than those of group B and group C (P 0.05). Conclusion The erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibition effects on the CFU- E and BFU-E growth of normal mice bone marrow cells. They may be low molecular-weight and medium-to-large molecular weight uremic toxins. High-flux dialyzer are more effective in clearing the erythropoiesis inhibitors than low-flux dialyzer.
【CateGory Index】： R692.5