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《China Poultry》 2019-17
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Molecular Evolution of miR-26 Gene Family and Its Expression Analysis in Chicken

HUANG Zhengyang;LI Shoufeng;WANG Qianbao;LI Chunmiao;HUANG Huayun;MU Chunyu;ZHAO Zhenhua;Poultry Institute,Chinese Academy of Agricultural Sciences;  
This experiment was conducted to investigate the miR-26 gene family evolutionary process and its expression regularity in different tissues.The sequences of miR-26 genes were searched in literature and miRBase.They were located by Ensemble database,and their phylogenetic tree was constructed by MEGA 10.0.In addition,the expression levels of miR-26 family were detected in different tissues and stages in chicken by RT-qPCR.A total 102 sequences in 34 species of vertebrate which indicated the extensive existence of miR-26 s(miR-26 a,miR-26 b,miR-26 c,miR-1297 and miR-4465) in different species.The analysis of gene localization showed all miR-26 genes located on the intergenic region.Besides,the similar expression of miR-26 s were detected in covary of pre-laying stage,laying stage and the egg laying peak stage.More miR-26 s mRNAs were detected at laying stage and the egg laying peak stage than that in pre-laying stage.It′s suggested that miR-26 s were highly conservative in evolution process and played a role in regulation reproductive activity in chicken.
【Fund】: 现代农业产业技术体系建设专项资金(CARS-41-Z05);; 2018省属公益类科研院所自主科研经费项目(BM2018026-2);; 扬州市自然科学基金项目(YZ2017105);; 扬州市现代农业项目(YZ2018045);; 江苏省家禽遗传育种重点实验室(JQLAB-ZZ-201705)
【CateGory Index】: S831
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Chinese Journal Full-text Database 1 Hits
1 LIU Yong-Ping;YANG Jing;LIU Yun;School of Chemical Engineering and Environment, North University of China;Research and Development Center for Integration of Green Agricultural Technology,Institute of Zoology,Chinese Academy of Sciences;;Research progress in microRNAs in insects[J];昆虫学报;2013-09
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Chinese Journal Full-text Database 1 Hits
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