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《China Poultry》 2019-17
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Rapid Identification of Duck Plague Based on Recombinase Aid Amplification Assay

XU Chao;DONG Xinji;WANG Chaoqun;ZHANG Juzhou;ZHANG Zihong;LI Ke;Technical Central of Zhengzhou Customs;Jiaozuo Customs;Xuchang Customs;  
According to the species specific gC gene of duck plauge virus,a general and fluorescence recombinase aided amplification assay were developed at temperature 39 ℃.The assay was able to accurately detect duck plauge virus and no cross reactivity with Muscovy duckling parvo virus,gosling parvovirus,avian infectious laryngotracheitis virus,Marek′s disease virus,Salmonella and Escherichia coli 0157 from duck.The sensitivity of this methord was up to100 copies/μL for general RAA and 10 copies/μL for fluorescence RAA,respectively.28 positive archieved samples and84 clinical samples were detected by RAA and commercial PCR kit.The results were 100% consistent with commercial PCR.Compared with classic PCR,the advantages of isothermal amplification at 39 ℃ or under room temperature,simple operation and speediness make RAA a promising molecular detection tool for duck plauge.
【Fund】: 国家质检总局科研计划项目(2016IK114)
【CateGory Index】: S858.32
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