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《中国药理学报(英文版)》 2002-06
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Probucol inhibits oxidized-low density lipoprotein-induced adhesion of monocytes to endothelial cells in vitro

LIU Ge-Xiu, OU Da-Ming, LI Li-Xin, CHEN Lin-Xi, HUANG Hong-Lin, LIAO Duan-Fang, TANG Chao-Shu' (institute of Pharmacy & Pharmacology, Nanhua University, Hengyang 421001, Hunan; Department of Cardiovascular Research , the First Hospital, Peking University, Beijing 100034, China)  
AIM: To investigate the mechanism by which probucol (PBC) affected adhesion of monocytes to human umbilical vein endothelial cells (HUVEC). METHODS:Effects of PBC on expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), P-selectin, and E-selectin in human umbilical vein endothelial cells were examined. Moreover, the inhibitory effect of PBC were compared with that of monoclonal antibodies (mAbs) to ICAM-1, VCAM-1, P-selectin, and E-selectin on adhesion induced by oxidized-low density lipoprotein ( Ox-LDL ). RESULTS: PBC at 10 to 80 μmol/L inhibited Ox-LDL-induced adhesion index from 16. 7 % to 7.0 % ( P 0.01 ) and Ox-LDL-induced expression of ICAM-1 (75 %) and P-selectin (72 % ). mAbs to ICAM-1 or P-selectin, when used alone, could only slightly reduce the adhesion of monocyte to HUVEC. When both monoclonal antibodies were used in combination, the adhesion was markedly inhibited from 16.7 % to 11. .3 % ( P 0.01), but the effect was still weaker than that of PBC (average 9.3 % ). CONCLUSION: PBC exerts its inhibitory effect on the adhesion of monocyte to HUVEC by inhibiting the expression of ICAM-1 and P-selectin.
【Fund】: Project supported by the National Natural Science Foundation of China. № 39970847 the Natural Science Foundation of Hunan Province № 99JJY20032 and the National Major Basic Research Program (973). № G2000056905.
【CateGory Index】: R96
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