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Isolation and Characterization of Total DNA from Several Fibre Crops and Their Allies

Guo Anping; Huang Ming;Zheng Xueqin; Peng Shiqing;Chen Xinqi and Li Yu(National Key Biotechnology Labor&tory for Tropical Crops, Chinese Academy of Tropical Crops (Dan Zhou,Hainan. 571737))(Institute of Bast Fibre Crops. Chinese Academy of Agricultural Sc  
AbstrsctA DNA extraction procedure namely SDS which had been developed by Pich and Schubery in 1993was improved and successfully used to obtain total DNA from Boehmeria. nivea (L ) Gasud, Hibiscus.cannabinus, Corchorus. olitorius, Corchorus. capsularis, Abutilon avicennae Gaertner and their -closespecies plants. The DNA yield, quality and purity were characterized, these isolated DNA could be digested with restiction enzymes and used for PCR amplification or RAPD analysis which are useful asmolecular genetyc makers. A fast, inexpensive procedure for extraction of high quality DNA from fibrecrops and their allies has been developed in this study,which is helpful for detecting the genetic diversityof those kind of plants in the field of DNA molecules.
【CateGory Index】: S563
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