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《CHINA'S FIBER CROPS》 1997-04
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Isolation and Characterization of Total DNA from Several Fibre Crops and Their Allies

Guo Anping; Huang Ming;Zheng Xueqin; Peng Shiqing;Chen Xinqi and Li Yu(National Key Biotechnology Labor&tory for Tropical Crops, Chinese Academy of Tropical Crops (Dan Zhou,Hainan. 571737))(Institute of Bast Fibre Crops. Chinese Academy of Agricultural Sc  
AbstrsctA DNA extraction procedure namely SDS which had been developed by Pich and Schubery in 1993was improved and successfully used to obtain total DNA from Boehmeria. nivea (L ) Gasud, Hibiscus.cannabinus, Corchorus. olitorius, Corchorus. capsularis, Abutilon avicennae Gaertner and their -closespecies plants. The DNA yield, quality and purity were characterized, these isolated DNA could be digested with restiction enzymes and used for PCR amplification or RAPD analysis which are useful asmolecular genetyc makers. A fast, inexpensive procedure for extraction of high quality DNA from fibrecrops and their allies has been developed in this study,which is helpful for detecting the genetic diversityof those kind of plants in the field of DNA molecules.
【CateGory Index】: S563
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【References】
Chinese Journal Full-text Database 10 Hits
1 HUO Guang et al(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205);Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L.Germplasm Resources Using ISSR Molecular Marker[J];Journal of Anhui Agricultural Sciences;2009-21
2 ZHOU Dong xin, QI Jian min,WU Wei ren, LI Wei ming (College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Studies on DNA extraction and establishment of RAPD reaction system in jute[J];Journal of Fujian Agricultural University(Natural Science);2001-03
3 BAI Zhan-bing,SU Jian-guang,GONG You-cai,CHEN Ji-quan,DAI Zhi-gang,WANG Feng-min(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,China);Genetic Diversity of Velvetleaf Germplasm Revealed by RAPD Marker[J];Hubei Agricultural Sciences;2008-02
4 HUO Guang, LI De-fang, CHEN An-guo, LI Jian-jun, TANG Hui-juan Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205;Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L. Germplasm Resources Using ISSR Molecular Marker[J];农业科学与技术(英文版);2009-03
5 ZHOU Jian-lin~1,JIE Yu-cheng~2 (1.College of Life Science,Hunan Normal University;Changsha 410081,China;2.Institute of Bast Fiber Crops,Chinese Academy of Agricultural Science,Changsha 410205,China);Isolation of Microsatellite loci in Kenaf(Hibiscus cannabinus)[J];Journal of Natural Science of Hunan Normal University;2006-01
6 CHEN Hua~1, XU Xiao-biao~(2*), YI Gan-jun~(3*),CAI Zu-guo~2 (1.Department of Biology, Zhaoqing College, Guangdong Province, Zhaoqing 526061, China; 2.College of Agronomy, JAU, Nanchang 330045, China; 3.Institute of Fruit Research, GAAS, Guangzhou 510640, China);A Study on Different Methods for Extraction of Kiwifruit Genomic DNA[J];Acta Agriculturae Universitis Jiangxiensis;2005-01
7 PAN Qi-hui, LAI Zhan-jun, SUN Xue-bing, LAI Xiao-Ping, HUANG Xiao-ying(Fibre Crops Institute of Jiangxi Province, Yichun 336000, China);Characteristics and Utilization of Wild Germplasm Resources in Boehmeria in China[J];ACTA AGRICULTURAE JIANGXI;2000-02
8 ZOU Yuan-xiu,LIAO Zhi-qiang,LAI Zhan-jun(Institute of Bast Fiber Crops of Jiangxi Province,Yichun 336000,China);Collection and Research Progress of Chinese Wild Plants in Boehmeria[J];Acta Agriculturae Jiangxi;2008-01
9 Guo Anping1 Zhou Peng1 Li Xiaoying1 Li Zongdao2 Zheng Xueqin1(1. National Key Biotechnology Laboratory for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 2. Hunan Agricultural University, Changsha 410128, China);RAPD Analyses of 17 Ramie (Boehmeria nivea ) Cultivars[J];Journal of Agricultural Biotechnology;2003-03
10 Guo Anping Zhou Peng Peng Shiqing Zheng Xueqin( National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101 );Factors Affecting the Reaction System of RAPD Analysis in Boehmeria Jacq.[J];Chinese Journal of Tropical Crops;2001-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Dai Silan, ChenJunyu, Gao Rongfu, Ma Jiangsheng, and Li Wenbin (Beijing Forestry University , 100083; The Institute of Genetics, Academia Sinica, Beijing 100101);DNA Isolation Procedure for RAPD Amplification Reaction of Nine Dendranthema Species[J];Acta Horticulturae Sinica;1996-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 FU Bing et al(Biotechnology Research Center of Kunming University of Science and Technolgy,Kunming,Yunnan 650224);Genetic Diversity Analysis of 25 Tobacco Germplasm Resources by ISSR Molecular Marker[J];Journal of Anhui Agricultural Sciences;2008-03
2 YANG Jia-hua et al(Qinghai University,Xining,Qinghai 810016);Research Progress on Heredity Diversity of Forage Germplasm Resources in China[J];Journal of Anhui Agricultural Sciences;2009-02
3 ZHAO Li-juan et al(Bio-technology Institute,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070);Analysis on Genetic Diversity in Common Buckwheat(Fagopyrum esculentum Moench) Based on ISSR Markers[J];Journal of Anhui Agricultural Sciences;2009-07
4 XIANG Yan 1,ZHU Su wen 2,CHENG Bei jiu 2 (1 College of Forest Utilization,Anhui Agricultural University ,Hefei 230036; 2.Biotechnological Centre,Anhui Agricultural University);Comparative Research on Several Isolation and Purification Method for Genomic DNA of Chinese Chestnut[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-01
5 HE Jin sheng,WANG Jian wei,WEN Le ying,XU Shu sheng,QU Jian guo,JIANG Hui ying,HONG Tao (Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China);The Systemic and Mucosal Immune Responses Can Be Induced Effectively by a Recombinant Adenovirus (Ad) Expressing Main Neutralizing Antigen VP7 of Group A Rotavirus[J];Chinese Journal of Virology;2001-02
6 Jin Shengjin Fang Zhaoyin Hang Changshou Wu Di(Institute of Virology,CAPM,Beijing,100052);CLONING AND EXPRESSION OF THE MAJOR CAPSID PROTEIN VP6 OF SIMIAN ROTAVIRUS STRAIN SA11[J];CHINESE JOURNAL OF VIROLOGY;1995-02
7 Li Guohua Jing Yu Qian Yuan (Capital Institute of Pediatrics,Beijing Municipal Laboratory of Infection and Immunity,Beijing 100020);SEQUENCE ANALYSIS OF THE GENE ENCODING VP7 OF A SEROTYPE 9 ROTAVIRUS FIELD STRAIN IDENTIFIED IN BEIJING[J];CHINESE JOURNAL OF VIROLOGY;1997-04
8 Li Guohua Qian Yuan Xiong Zhaohui Jing Yu (Beijing Municipal Laboratory of Infection and Immunity,Capital Institute of Pediatrics,Beijing 100020);SEQUENCE ANALYSIS OF THE GENES ENCODING VP7 PROTEINS FROM FOUR HUMAN ROTAVIRUS FIELD STRAINS REPRESENTING G1-G4 SEROTYPES[J];CHINESE JOURNAL OF VIROLOGY;1998-02
9 He Xiangjun Qian Yuan Jing Yu Li Guohua (Capital Institute of Pediatrics, Beijing Municipal Laboratory of Infection and Immunity, Beijing, 100020);EXPRESSION OF TYPE G1 VP7 FROM A GROUP A ROTAVIRUS FIELD STRAIN IN BACULOVIRUS SYSTEM[J];CHINESE JOURNAL OF VIROLOGY;1999-01
10 WANG Yong\|Qing\ XU Lai\|Xiang\ ZHANG Zhi\|Bin (National Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, The Chinese Academy of Sciences Beijing\ 100080,China);The Standardization of RAPD[J];CHINESE JOURNAL OF ZOOLOGY;2000-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Xu Haixia,Zhang Xia,Yan Ping,Du Jinzhou(College of Biological Engineering,Shihezi University,Shihezi,Xinjiang 832003,China);A Studies on DNA Extraction and Establishmen of RAPD Reaction System in Achnatherum splendens[J];Anhui Agricultural Science Bulletin;2007-09
2 Mao Yanzhi(Potato Research Institute;Heilongjiang Academy of Agricultural Sciences;Keshan;Heilongjiang 161606);Rearch Progress of Transgene CP Potato Breeding for Resisting against Viruses[J];Anhui Agricultural Science Bulletin;2008-03
3 Chen hong et al.(Hainan university,Hainan Haikou,571101);Research progress on application of biotechnology to agave spp[J];Anhui Agricultural Science Bulletin;2008-15
4 YANG Gu-liang et al(Huanggang Normal College,Huanggang,Hubei 438000);Comparison of RNA Extraction from Ginkgo biloba L.Leaf and RT-PCR[J];Journal of Anhui Agricultural Sciences;2007-12
5 LIN Zhi-hua et al(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225);Study on Extraction of Actinidia Genomic DNA[J];Journal of Anhui Agricultural Sciences;2009-03
6 HUO Guang et al(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205);Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L.Germplasm Resources Using ISSR Molecular Marker[J];Journal of Anhui Agricultural Sciences;2009-21
7 YANG Ke-jin,LU Yun-feng,ZHANG Jing(College of Life Science and Technology,Nanyang Normal University, Nanyang,Henan 473061,China);A Study on Five Methods for Extraction of Pelargonium Genomic DNA[J];Northern Horticulture;2009-06
8 ZHU Xiu-zhi~1,XIANG Cheng-hua~2,PENG Zheng-song~1,YANG Jun-bao~1,LUO Cheng-ke~3 (1.College of Life Science,China West Normal University,Nanchong 637002,China;2.Sichuan Academy of Forestry,Chengdu 610000,China;3.Ningxia University,Yinchuan 750021,China);Genomic DNA extraction and optimization of RAPD analytic conditions of Michelia wilsonii[J];Journal of Agricultural University of Hebei;2006-02
9 Yu Xiaoli(Department of Biology and Pharmacy Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China); Fan Ximei(Jiaozuo Teachers College); Liu Shi(Senior High School of Xiayi County);Methods for Extraction of Genomic DNA from Rosa banksiae Aiton[J];Journal of Northeast Forestry University;2007-08
10 GUO Jinping, PAN Daren, XU Liping, YUAN ZhaonianChina, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Establishment of DNA fingerprint and its application to variety identification in sweet potato[J];;2003-01
China Proceedings of conference Full-text Database 1 Hits
1 CHEN Rong-bing LIN Zheng-he CHEN Chang-song YOU Xiao-mei (Tea Research Institute,Fujian Acaedemy of Agricultrual Sciences, Fu’an Fujian 355014,China);Assessment of Genetic Diversity of Tea Detected by RAPD and ISSR[A];[C];2007
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