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《Plant Fiber Sciences in China》 2009-06
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Tissue Culture and Hairy Roots Inducement of Apocynum venetum

LIU Xiao-rui,JI Qiao-ling(College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)  
Objective:culture of callusand inducement of hairy roots of Apocynum venetum.Methods: Callus derived from seeds of A.venetum were amplified on a series of mediums,optimal medium was screened for propagation of the callus which is harder and with more meristematic cells.The callus was cultured in liquid medium for exploring the conditions of hairy roots inducement.Results:MS+0.5mg/L 6-BA+0.3 mg/LNAA+300mg/L LH was the most appropriate medium for the callus propagation,culture temperature was 24 ±2℃,illumination was 1500~2000Lx and photoperiod was 12h light/12h dark.1/2MS+0.1mg/LNAA+35g/L sucrose was the optimal liquid medium for production and growth of hairy roots,the culture conditions were at 17-200C,rotation speed was 60rpm in dark and 40~42d was a cycle of renewing medium.Low temprature was the necessary to rooting.These results laid the foundation for the establishment of a large-scale culture system of A.venetum hairy roots and the reseach for metabolic control of the main secondary metabolites.
【Fund】: 国家自然科学基金项目(亚麻与罗布麻体细胞杂交及其种质的耐盐性遗传研究30660095);; 新疆教育厅重点科研项目(亚麻与罗布麻体细胞杂交育种研究XJEDU2006108)
【CateGory Index】: S563.7
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