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《China Vegetables》 2013-06
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Studies on Potato Transformation of BDN1 Gene Mediated by Agrobacterium tumefaciens

PAN Ying-xue 1,ZHANG Li-li 1*,SHI Ying 1,LU Cui-hua 1,LIN Zhong-ping 2(1 College of Agronomy,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;2 College of Life Science,Peking University,Beijing 100871,China)  
The BDN1 gene in coding area from drought-tolerant Boea crassifolia Hemsl was transferred into potato(Solanum tuberosum L.)cultivars' Dongnong 303'through agro-bacteriummediated transformation.The factors influencing its genetic transformation were studied.Thirty-five transgenic plants with kanamycin resistance were obtained.Specific primers were used to amplify the full length of the BDN1 gene from total genomic DNA of the transgenic plants.Among them,27 plants were positive by PCR test and 4 plants were positive by Southern-blot test.The result of semi-quantitative RT-PCR test showed that BDN1 gene was inserted into the genome of the transgenic plants and can be transcribed normally.The physiological index showed the transgenic potato plants were significantly tolerant to drought stress.
【Fund】: 黑龙江省科技计划项目(GZ11B103);; 国家高技术研究发展“863”计划项目(2009AA10Z103)
【CateGory Index】: S532
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