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《China Vegetables》 2017-10
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Effect of Environmental Temperature on Nitrate Content of Pakchoi,NO_3~--N Content in Soil and Activity of Key Enzymes in Nitrogen Metabolism

ZHANG Chun-lai;YANG Yun;XU Wei-hong;CHI Sun-lin;WANG Wei-zhong;LI Yan-hua;LI Tao;College of Resources and Environmental Sciences,Southwest University;  
The effects of different temperature(5,15,25℃)on biomass,nitrate content,activities of keyenzymes,soil nitrate nitrogen content,and key enzymes activities of plant-soil nitrogen metabolism of2 pakchoi varieties[Brassica campestirs L.spp.chinensis(L.)Makino var.communis Tsen et Lee](lownitrate variety‘Jienong No.4’and high-nitrate variety‘Xianggangtexuan’)were studied under artificial climate incubator by pot experiment.The results indicated that 15℃was the optimal temperature for growth of 2 pakchoi varieties.The shoot dry weight of‘Jienong No.4’and‘Xianggangtexuan’increased by 23.2%and 26.8%,respectively.The nitrate contents in leaves of 2 pakchoi varieties were increased significantly along with the increase of temperature.Compared with the treatment 5 ℃,the nitrate content in leaves of‘Jienong No.4',‘Xianggangtexuan'under the light intensity of 15 ℃ and 25 ℃ increased by 15.0% and 18.3%,6.7% and 16.7%,respectively.The activity of nitrate reductase(NR)in leaves of pakchoi increased with temperature increasing.The activity of nitrite reductase(Ni R)in leaves of pakchoi increased first,then decreased.The activities of glutamate synthase(GOGAT) in leaves of pakchoi decreased.With the increase of temperature,the soil NO_3~--N content of‘Xianggangtexuan'increased,while soil NO3--N content of‘Jienong No.4'decreased. The activity of nitrate reductase in soil decreased first,then increased with the increase of temperature.But the urease activity in soil increased at first and then decreased.Under 3 temperature treatments,nitrate contents in leaf and petiole of‘Jienong No.4'were lower than that of‘Xianggangtexuan'.
【Fund】: 国家现代农业产业技术体系建设专项(Nycytx-23);; “十一五”国家科技支撑计划项目(2007BAD87B10)
【CateGory Index】: S634
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