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Genomic RAPD Analysis and Comparative Study on Field Voles (Microtus Fortis)from Two Regions in China

WANG Shengchang,XIE Jianyun,SHAO Weijuan,GAO Cheng (Shanghai Quality Monitoring Center For Laboratory Animals,Shanghai 200032,China)  
Objective To compare and analyze genetic characteristics of field voles(Microtus fortis)in DNA level,and to distinguish the field voles by RAPD markers Methods Six pieces of 10bp single random primers were selected and used to amplify genomic DNA of Microtus fortis from two regions in China Results 20 fragments of genomic DNA were shared by the field voles from two regions Two pieces of primers could be used as characteristic RAPD markers The genetic background of individuals from two regions was not homogeneous at RAPD markers Conclusion RAPD could be used to distinguish the species(or subspecies) of Microtus fortis from the two regions in DNA level
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