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《Acta Laboratorium Animalis Scientia Sinica》 2014-02
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Pathological changes of the kidneys in mouse models of adriamycin-induced-nephrosis

LIU Lian;ZHANG Gao-fu;LI Qiu;WANG Mo;Department of Nephrology and Immunology,Children's Hospital of Chongqing Medical University,Ministry of Education Key Laboratory of Child Development and Disorders,Key Laboratory of Pediatrics in Chongqing,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders;  
Objective Our purpose was to observe the renal pathological changes in the mouse modells of adriamycin-induced nephropathy in different periods. Method 48 healthy male BALB / c mice were randomly divided into control group and model group. The model group received a disposable tail vein injection of adriamycin 10. 5 mg / kg body weight, and the control group received the same amount of saline. 24-hour urinary protein,serum biochemical indexes and kidney pathological changes were dynamically observed for 12 weeks. Results Proteinuria of model mice appeared in the 2th week after ADR injection,which lasted to the end of the 12-week experiment,At the 8th week,the amount of urine protein reached a peak( P 0. 05); The serum albumin was decreased at the 4th week,cholesterol was increased at 8th week. At the end of experiment,serum creatinine was also increased( P 0. 05). Minimal change nephrotic syndrome( MCNS) was observed in model mice at the 4th week; the lesions in renal tissues at 8th weeks were more serious than that at 4th weeks, but glomerular sclerosis was unconspicuous. Focal segmental glomerulonephritis( FSGS) was seen at the 12th week. The GSI of the model mice was( 2. 81 ± 0. 84) %,significantly higher than that of the control mice(( 0. 33 ± 0. 21) %) at 12thweek( P 0. 01). Conclusions A mouse model with adriamycin-induced-nephrosis can be successfully established by a disposable tail vein injection of adriamycin in a dose of 10. 5 mg / kg body weight. The early manifest ation of this model is MCNS,and at a late stage,it may be changed into FSGS.
【Fund】: 国家自然科学基金资助青年项目(No.81100506)和国家自然科学基金资助面上项目(No.81270802)
【CateGory Index】: R726.9
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