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《Chinese Journal of Rice Science》 2012-04
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Silencing of OsDUF500 Gene in Rice Enhances Resistance to Xanthomonas oryzae pv. oryzae

LI Juan,CHANG Shan-shan,LIU Feng-quan,SHAO Min (Key Laboratory of Monitoring and Management of Plant Diseases and Insects,Ministry of Education,Nanjing Agricultural University,Nanjing 210095,China;)  
Conserved functional region of DUF500 was cloned by PCR from cDNA of expression HarpinXoo(hrf1) transgenic rice NJH12.OsDUF500 encodes an unknown function member of DUF500 family.Calli from mature embryos of rice cultivar R109 were transformed by Agrobacterium tumefaciens strain EHA105/ABD::pVec8.There were eight OsDUF500 transgenic lines from nine hygromycin resistant lines by PCR analysis.Compared with the wild type,the down-expressed lines ABD are characterized by reduced plant height,shortened leaf and white blighted grain.Disease assays of transgenic rice plants infected by Xanthomonas oryzae pv.oryzae(PXO99) indicated that different transgenic lines had different resistance to rice leaf blight.Lesion length of the down-expression lines reduced significantly and lesion area all bellowed 30%.Lesion areas of the ABD-3 and ABD-5 were 12.3% and 15.7%,respectively.Thus,we predicted OsDUF500gene,which was up-regulated in rice blast,maybe was a negative regulator on bacterial blight resistance.
【Fund】: 国家863计划资助项目(2008AA10Z108 2007AA10Z188);; 国家转基因生物新品种培育重大专项(2009ZX08001-005B)
【CateGory Index】: S511
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