Effects of HCN2 in the development of peripheral neuropathic pain in rats
HUANG Tao;FU Bo;WANG Jing;WANG Bin;LIU Shao-jun;WENG Xie-chuan;Department of Biology,Institute of Basic Medical Science,Academy of Military Medical Science;State Key Laboratory of Proteomics,Academy of Military Medical Sciences;Institute of Health and Environmental Medicine,Academy of Military Medical Sciences;
Objective: To explore the effects of hyperpolarization-activated cyclic nucleotide-gated channels 2( HCN2) in the formation of peripheral neuropathic pain in rats. Methods: Twenty-four healthy adult rats were divided into two groups randomly( n = 12) :the sham group rats were only isolated the left L4,L5 spinal nerve,the spinal nerve ligation( SNL) group was separated the spinal nerve and performed the corresponding ligation. The behavioral experiments were tested 7 days after operation; The model rats were randomly divided into 3 groups( n = 6) :(1) negative group( Saline),intra-plantar injection of saline in left hindpaws;(2) positive group( gabapentin,GBPT),intraperitoneal injection of gabapentin;(3) experimental group( ZD7288),intra-plantar injection of ZD7288 in left hindpaws. The behavioral experiments were tested before injection and 1 h,4 h,24 h and 48 h after injection; Obtaining the dorsal root ganglion( DRG) of the control group( before operation),sham group and the SNL group( n = 6),using q PCR and Western blot to analyze the mRNA and protein of HCN2 in rats' DRG. Results: The rat model of neuropathic pain was successfully established. Compared with saline group,GBPT group and ZD7288 group could significantly reduce the symptoms of neuropathic pain in rats after injection 1 h( P 0. 01),and there was no difference between GBPT group and ZD7288 group. Compared with control group and sham group,the expression of HCN2 mRNA in SNL group's DRG was significantly increased( P0.01),and the expression of HCN2 channel protein was also increased significantly( P 0. 05). Conclusion: HCN2 is involved in the development of peripheral neuropathic pain and is likely to be a potential new target for the treatment of neuropathic pain.
【Fund】： 北京市自然科学基金面上项目(7142123);; 蛋白质组学国家重点实验室优青课题(SKLP-YB201403);; 总装预研基金课题(9140A26060215JB94001)
【CateGory Index】： R741
【CateGory Index】： R741