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《Journal of Chinese Institute of Food Science and Technology》 2011-01
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HPLC Fingerprints in Distinguishing Propolis and Poplar Tree Gum

Zhang Cuiping Hu Fuliang*(College of Animal Sciences,Zhejiang University,Hangzhou 310029)  
Objective:To establish fingerprints method to differentiate between propolis and poplar tree gum by HPLC.Methods:The HPLC method was adopted to detect the samples extracted with 95% ethanol.The separation was performed on Agilent Eclipse XDB-C18(4.6 mm×250 mm,5 μm) with the mobile phase consisted of methanol and 0.5% H3PO4 at a 0.6 mL/min flow rate,a 268 nm detection wavelength and a temperature of 30 ℃.Results:The fingerprint of propolis collected from different places showed the similar characteristics,but they were different from those of poplar tree gum.The chromatographic finger prints of eight propolis samples from different producing areas have two characteristic peaks,while the poplar tree gums don't have these peaks.However,3 characteristic peaks,not obtained in propolis samples,appear in three poplar tree gum samples from different areas.Furthermore,these five characteristic peaks are distinct and easy to identify,with no interference among each other.Conclusions:The method is both repeat and accurate,which can be used to distinguish poplar tree gum from propolis and to detect the adulteration of poplar tree's gums in propolis.
【Fund】: 国家蜂产业技术体系建设专项资金资助项目(NYCYTX-43);; 浙江省自然科学基金资助项目(R3090332)
【CateGory Index】: TS207.3
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