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《Journal of Desert Research》 2005-05
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Genetic Diversity and Relationships among Species of Caragana Fabr.

SHENG Hong-mei 1, CHEN Tuo 2, AN Li-zhe 1,2, ZHEN Xiao-ling 1, PU Ling-ling 1, LIU Ya-jie 1, SUN Xue-gang 3(1.Key Laboratory of Arid and Grassland Agroecology, Lanzhou University, Ministry of Education, Lanzhou 730000, China; 2.Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China; 3.Gansu Agricultural University, Lanzhou 730070, China)  
RAPD markers were used to assess the genetic diversity of Caragana Fabr. and to estimate genetic relationships among the eleven species growing in Gansu province. The 11 primers were screened from 34 10-bp arbitrary primers, and a total of 128 loci were amplified; the length of fragments was between 200 bp and {2 000} bp, among which, 104(81^25) were polymorphic. The Shannon's information index and Nei s gene diversity were 0^3889 and 0^2517, respectively. The results showed that the Caragana Fabr. had a high level genetic diversity, and its adaptability to environmental change was strong. A dendrogram based on genetic distances was constructed by UPGMA method of POPGENE version 1^32. The conclusion was almost consistent with the results of traditional morphologic taxa, except hypotaxis status of very few species was slightly changed. It indicated that it is necessary for further studying their relative relations to combine molecular markers with morphologic and other methods.
【Key Words】: Caragana Fabr. RAPD genetic diversity genetic relationship
【Fund】: 国家自然科学基金委重大研究计划项目(90302010);; 中国科学院“西部之光”项目;; 甘肃省农业生物技术研究与应用开发项目;; 国家转基因植物研究与产业化开发专项(JY03A23)共同资助
【CateGory Index】: Q943
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