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《Chinese Veterinary Science》 2010-05
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Effects of the standardized purified dry extract Polinacea~(TM) from Echinacea angustifolia on immunity function of peripheral blood mononuclear cells of dairy heifers

WU Hua1,Alessandro Nardone2,Nicola Lacetera2 (1.College of Agriculture and Animal Husbandry,Qinghai University,Xining 810003,China; 2.Tusica University,Viterbo 01100,Italy)  
PBMC from six Holstein heifers were incubated with 0,6.3,20,60,or 180μg/mL of the experimental compound PolinaceaTM from Echinacea angustifolia with or without concanavalin A(Con A,1μg/mL)or pokeweed mitogen(PWM,0.6μg/mL),respectively.Proliferation of PBMC was assessed by ELISA,and secretion of IFN-γ was stimulated by ConA(2.27μg/mL).In result,the lowest and highest increase of proliferation were observed with PolinaceaTM at 6.3(2-fold increase) or 180(10-fold increase)μg/mL,respectively.PolinaceaTM at 180μg/mL reduced ConA-driven proliferation,whereas at 20 and 60μg/mL improved proliferation of PWM-stimulated PBMC.However IFN-γ secretion was not affected by PolinaceaTM.In conclusion,PolinaceaTM could modulate proliferation of bovine PBMC,and deserve to be tested in vivo to define conditions that might benefit from its utilization.
【CateGory Index】: S567.239
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