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《Chinese Veterinary Science》 2011-12
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Study on antivirus activity of crude extract from neem seed kernel against duck plague virus in vitro

XU Jiao1,SONG Xu1,YIN Zhong-qiong1,2,CHENG An-chun1,2,JIA Ren-yong1,2,LIU Yin-zhi1, YE Kui-chuan1,DENG Yun-xia1(1.College of Veterinary Medicine,Sichuan Agricultural University,Ya'an 625014,China; 2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Ya'an 625014,China)  
The three fractions obtained from chloroform extracts of ethanol extract of neem(Azadirachta indica) seed kernel by petroleum ether,ethyl acetate and distilled water were investigated for antivirus activity against duck plague virus(DPV)in vitro.Duck embryo fibroblasts infected with DPV were treated with the drugs we prepared and the effect of antivirus was judged by cytopathic effect(CPE) and 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT) colorimetric method.The results showed that when the concentrations were 0.000 5,0.000 3 and 0.156 0 mg/mL,the virus inhibition rates of the three extracts were 13.23%,29.24% and 11.72%,respectively,and the maximum non-toxic concentrations of the three extractives were 0.007 8,0.003 9 and 0.125 0 mg/mL respectively.
【Fund】: “十二五”农村领域国家科技计划项目(2011BAD34B03-4);; 四川省科技支撑计划项目(2010N20043);; 教育部“长江学者和创新团队发展计划”创新团队项目(IRT0848)
【CateGory Index】: S853.7
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