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Development of Immunoassay Methods for two β 2 Agonists

Chen Jiming Han Zhengkang Gong Xiaoming Lu Chengping Du Nianxing (College of Veterinary Medicine, Nanjing Agric Univ, Nanjing, 210095)  
Two β 2 agonists, Clenbuerol (CL) and Cimaterol (CIM) were conjugated with carrier proteins by diazotization and EDC respectively. These conjugates were used to immunize rabbits to prepare anti CL and anti CIM sera. The titers and the specificities of the sera were detected by indirect ELISA and inhibitory indirect ELISA. Then the sera were used to build up immunoassay methods for the two kinds of β 2 agonists. The work dilutions of two kinds of sera were 1∶10 000 and 1∶5000 respectively and the sensitivites of detection ranged from 1.9 ng to 15.6 μg and from 0.477 ng to 1.85 μg respectively. These immunoassay methods could be used to examine the overdue usage of CL and CIM in animal productions and to detect the residue of CL and CIM in meat products.
【Fund】: 国家自然科学基金
【CateGory Index】: S859.793
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