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《Sugar Crops of China》 2009-02
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Study on Virus-free Culture Techniques of Sugarcane Stem Tip

LIU Li-min,LI Song,DAI You-ming,YU Kun-xing,LIU Hong-jian,DAN Ming(Guangxi Sugarcane Research Institute,Nanning 530007,China)  
The experiment were undertaken to solve the problems of high contamination rate and severe phenol pollution in hot water treatment with stem tip culture to product sugarcane virus-free seedlings.The experimental results showed that it was need for different temperatures and time to different sugarcane varieties in the hot water treatment;75% alcohol pretreatment before stem tip dissection reduced the contamination rate;the phenol pollution was reduced when stem tip was dipped in axenic water or 150 mg/L PVP solution for 15 min.PVP also could promote the growth of the stem tip.There were both positive and negative effects to stem tip growth when added AC as medium,but the further experiment is need.
【Fund】: 广西农业科学院技术发展资金(2006006)
【CateGory Index】: S566.103
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