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《Chinese Journal of Eco-Agriculture》 2008-04
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Screening of phytase producing strain and optimization of fermentation conditions

YANG Yan-Ling1,FANG Jin-Tong1, HUANG Tian-Sun2(1.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Wuyishan Institute of Agricultural Sciences, Wuyishan 354300, China)  
A phytase-producing strain was isolated and identified as Aspergullus niger FZ41. Liquid fermentation was investigated and the optimal fermentation medium obtained. A 500 mL flask filled with 50 mL inoculums with an initial pH of 6.0 was used in the inoculation. The relative percents of ingredients of fermentation medium were: starch 4%, NH4NO3 0.08%, KCl 0.05%, MgSO4·7H2O 0.05%, FeSO4·7H2O 0.003%, MnSO4·7H2O 0.003%. The age of the inoculums was 36 h, with initial medium pH 6.0 and an inoculation dose of 5%. Extra-cellular phytase production was 219 U·mL-1 under fermentation conditions of 30 ℃ at 220 r·min-1 for 84 h.
【Fund】: 福建省科技计划重点项目(2007N0094);; 福建省自然科学基金项目(2007J0243 X0750005);; 福建省教育厅科技计划项目(JA07059)资助
【CateGory Index】: S816
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