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《Chinese Journal of Microecology》 2008-03
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Identification and species analysis of the Lactobacillus heilongjiang strain from the intestinal tracts of healthy piglets

LIU Yu1,ZHU Zhan-bo1,ZHAO Xing-cheng2,XI Ying1,WANG Hong-wei1,CUI Yu-dong3,YANG Huan-min1(1.Department of Animal Science and Technology,Heilongjiang August First Land Reclamation University,Daqing 163319,China;2.Heilongjiang Province Beidahuang Meat Industy Co.Ltd.Baoquanling Subsidiary,Baoquanling 154211,China;3.Department of Life Science and Technology,Heilongjiang August First Land Reclamation University,Daqing 163319,China)  
To isolate and screen Lactobacillus strains from the intestinal tract of healthy piglets for micro-ecological agents′s manufacture.64 faecal samples were obtained in piglets of 12~60 age from pig farms in Harbin,Daqing and Baoquanling of Heilongjiang Province.The faecal samples were cultivated on MRS culture,48 strains were identified as Gram-positive,anaerobic and nonspore-bearing bacillus through the observation of colonial morphology and cultural character.Then,18 strains were defined eventually as Lactobacillus through the physiological and biochemical identification and PCR assay.7 strains were defined as L.reuteri,5 strains as L.acidophilus,2 strains as L.johnsonnii,1 strain as L.brevis,1 strain as L.casei subsp.pseudoplantarum,1 strain as L.plantarum and 1 strain as L.jensenii.
【Fund】: 黑龙江省农垦总局科技计划课题(HNKXIV-08-03-06);; 黑龙江省科技厅国际科技合作项目(WB06B06)
【CateGory Index】: S852.6
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