EXPERIMENTAL OBSERVATION ON EFFICACY OF COMPOUND CHLORINE DIOXIDE DISINFECTANT IN KILLING MICROORGANISMS IN WATER

Shi Laishun Xie Chaoren ( Chemical Industry Department, Shandong Industrial University, Jinan 250014 )  

The LX-type chlorine dioxide water solution generator was used to prepare the compound solution of chlorine dioxide and chlorine (abbreviated to compound chlorine dioxide disinfectant below) containing available chlorine 3 246 mg/L. The compound solution was used to observe experimentally its efficacy in killing the microorganisms in water and to examine the stability of its available chlorine and its acute toxicity in mice. The results indicated that a killing rate of over 99.9% was achieved when Staphylococcus aureus or Escherichia coli was exposed to a solution containing available chlorine 4.5 mg/L for 2 min, Candida albicans was exposed to a solution containing available chlorine 5.0 mg/L for 1 min, or spores of Bacillus subtilis var. niger was exposed to a solution containing available chlorine 90.0 mg/L for 5 min. A solution containing available chlorine 200.0 mg/L with a 60-minute contact time or a solution containing available chlorine 400.0 mg/L with a 5-minute contact time could destroy the antigenicity of HBsAg. For killing the spores of B. subtilis var. niger in suspension containing 10% calf serum, a solution containing available chlorine 180.0 mg/L with a 20-minute contact time was required to obtain a killing rate of over 99.9%. When the compound chlorine dioxide disinfectant solution held in a white plastic vessel or in a glass bottle was stored at room temperature in a place shielded from the light for 1 week, its available chlorine content decreased by 23 81% and 7.19% respectively, while the rate of decrease in available chlorine was as high as 73 99% if the solution was stored in a glass bottle at a place not shielded from light. The compound chlorine dioxide disinfectant had an oral LD 50 of higher than 10 000 mg/kg in mice and, therefore, belongs to actually nontoxic group.

【Key Words】： water disinfection chlorine dioxide HBsAg Candida albicans Bacillus subtilis var. niger Escherichia coli Staphylococcus aureus
【CateGory Index】： R187.2

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