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《China Cucurbits and Vegetables》 2011-04
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Establishment of Genetic Transformation System in Radish

LI Hai-ping,ZHANG Lu-gang,XU Yu-jian,FAN Ai-li(College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi,712100,China)  
The factors for Agrobacterium tumefaciens-mediated genetic transformation in Radish(Raphanus sativus L.)were studied.A radish genetic transformation system is established.Cotyledons with petiole were cultured in MS + 6-BA 6 mg·L-1 + NAA 0.05 mg·L-1 for 2 days as pre-culture.They were then infected for 5-7 min by the EHA105 Agrobacterium at OD600 = 0.3-0.5,and co-cultured in same medium for 5 days.The explants were then transferred to MS + 6-BA 6 mg·L-1 + NAA 0.05 mg·L-1 + 500 mg·L-1 Cef medium for 7 days for pre-screening.The explants were later cultured no MS + 6-BA 6 mg·L-1 + NAA 0.05 mg·L-1 + 500 mg·L-1 Cef + 5 mg·L-1 Hyg medium for 10 days for selecting transgenic events.We got 126 resistant shoots,and 8 of them were amplified the target gene BcA9 with size about 850 bp.The preliminary validation showed that the target gene has been transferred into radish.
【Fund】: 国家科技支撑计划项目(2009BADB8B03-1)
【CateGory Index】: S631.1
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