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《China Cucurbits and Vegetables》 2011-04
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Establishment of Genetic Transformation System in Radish

LI Hai-ping,ZHANG Lu-gang,XU Yu-jian,FAN Ai-li(College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi,712100,China)  
The factors for Agrobacterium tumefaciens-mediated genetic transformation in Radish(Raphanus sativus L.)were studied.A radish genetic transformation system is established.Cotyledons with petiole were cultured in MS + 6-BA 6 mg·L-1 + NAA 0.05 mg·L-1 for 2 days as pre-culture.They were then infected for 5-7 min by the EHA105 Agrobacterium at OD600 = 0.3-0.5,and co-cultured in same medium for 5 days.The explants were then transferred to MS + 6-BA 6 mg·L-1 + NAA 0.05 mg·L-1 + 500 mg·L-1 Cef medium for 7 days for pre-screening.The explants were later cultured no MS + 6-BA 6 mg·L-1 + NAA 0.05 mg·L-1 + 500 mg·L-1 Cef + 5 mg·L-1 Hyg medium for 10 days for selecting transgenic events.We got 126 resistant shoots,and 8 of them were amplified the target gene BcA9 with size about 850 bp.The preliminary validation showed that the target gene has been transferred into radish.
【Fund】: 国家科技支撑计划项目(2009BADB8B03-1)
【CateGory Index】: S631.1
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 WANG Binglin1,LI Yuanyuan2,HAN Taili1(1.Weifang Academy of Agricultural Sciences,Shandong 261061;2.College of Biological Engineering,Weifang University);Progress and Prospect of Molecular Biology in Radish in China[J];Journal of Changjiang Vegetables;2009-08
2 XU Wei-rong,WANG Yue-jin~*,WANG Xi-ping,HAO Wei,SUN Ma(College of Horticulture,Northwest Sci-Tech University of Agriculture and Foresina Agricultural Ministry,Yangling,Shaanxi 712100,China);Construction of the Plant Expression Vectors Carrying Resistant Genes to Powdery Mildew and Adversities inWild Species of Vitis in China[J];Acta Botanica Boreali-occidentalia Sinica;2005-05
3 Liu Fan Yao Lei Li Yan Cao Mingqing (Beijing Vegetable Research Center,Beijing 100081)Liu Gongshe (Institute of Botany,Chinese Academy of Science,Beijing 100093)C Robaglia C Tourneur (Laboratoire de Biologie Cellulaire,INRA,78026 Vers;Transformation of Chinese Cabbage via Cocultivation of Microspore Derived Embryos with Agrobacterium tumefaciens[J];ACTA AGRICULTURAE BOREALL—SINICA;1998-04
4 YANG Guang Dong ZHU Zhen LI Yan Oe ZHU Zhu Jun (Department of Horticulture ,Zhejiang University, Hangzhou 310029);TRANSFORMATION OF Bt-CpTI FUSION PROTEIN GENE TO CABBAGE (BRASSICA OLERACEA VAR. CAP/TATA)MEDIATED BY AGROBACTERIUM TUMEFACIENS AND PARTICLE BOMBARDMENT[J];Acta Biologiae Experimentalis Sinica;2002-02
5 HE YUKE(Department of Horticulture, Northwestern Agricultural University, Yang-Ling, Shaanxi, China)ROEIAND E. VOORRPS(Institute for Horticultural Plant Breeding,Wageningen,The Netherlands);High Transformation Freqtuncy of Brassica Crops with Agrobacterium rhizogenes[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;1989-04
6 ZHU Chang xiang, SONG Yun zhi, ZHANG Song, GUO Xing qi , WEN Fu jiang (College of Life Sciences, Shandong Agricultural University, Taian 271018,China);PRODUCTION OF TRANSGENIC CHINESE CABBAGE BY TRANSFORMATIONWITH THE CP GENE OF TURNIP MOSAIC VIRUS[J];Acta Phytopathologica Sinica;2001-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Li et al (Beijing Vegetable Research Center, Beijing 100089);Progress of Haploid Breeding in Cruciferae Crops[J];Journal of Anhui Agricultural Sciences;2003-02
2 Wu Xiaoliang et al (College of Agronomy and Life Sciences Southwest Agricultural University, Beibei, Chongqing 400716);Advances of the Studies on Engineering in Rape Breeding[J];Journal of Anhui Agricultural Sciences;2004-01
3 SUN Lei et al (National Flower Engineering Technology Research Center,College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Application of Thin Cell Layer Technology in Propagation and Genetic Transformation of Ornamental Plant[J];Journal of Anhui Agricultural Sciences;2007-22
4 XU Ben-bo et al (Chongqing Rapeseed Engineering Research Center,College of Agronomy and Biotechnology,Southwest University,Chongqing 400716);Influence of Co-culture Media on Transient Expression of GUS Gene and Transformed Efficiency of Hypocotyls of Rapeseed(Brassica napus L.) Mediated by Agrobacterium tumefaciens[J];Journal of Anhui Agricultural Sciences;2007-25
5 LI Ji-tao et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101);Advances in Studies of Seed-specific Promoters[J];Journal of Anhui Agricultural Sciences;2008-04
6 Peng Binghui, Wang Xiufeng;Progress and Security Analysis of Transgenic Technology in Application of Vegetable Breeding[J];Journal of Changjiang Vegetables;2005-06
7 GUAN Feng, YANG Lianyong, JIANG Shouquan, WANG Li ( Institute of Seed Science, Hunan Hejiashan Original Breeding Farm, Changde, Hunan 415123 );Research Progress of Breeding and Cultivation in Brassica juncea Coss. var. tumida Tsen et Lee[J];Journal of Changjiang Vegetables;2009-22
8 HU Jing1,MA Hui-ling1,XIE Jun-ren2,GENG Xiao-li1 (1.College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China);Evaluation of two antibiotics and their effect on tissue culture of Medicago sativa cv. Gannong No.3[J];Grassland and Turf;2007-02
9 PAN Chunqing1,ZHANG Junhua1,CUI Chongshi2,WEN Jingzhi1 (1. College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2. College of Horticulture, Northeast Agricultural University, Harbin 150030, China);Current study on the turnip mosaic virus[J];Journal of Northeast Agricultural University;2008-01
10 ZHANG Jin-wen~1,FAN Xing-zhong~2,WANG Ying~2,WANG Han-ning~(1*)MENG Ya-xiong~1,LONG Feng~3(1 College of Agronomy,Gansu Key Laboratory of Crop Genetic Improvement Sci-Tech Germplasm Enhancement,Gansu Agricultural University,Lanzhou 730070,China;2 Jiuquan Agricultural Research Institute,Jiuquan,Gansu 735000,China;3 College of Life Science,Lanzhou University,Lanzhou 730000,China);In Vitro Culture and Regeneration System of Pepper(Capsicum annuum)[J];Acta Botanica Boreali-Occidentalia Sinica;2006-09
China Proceedings of conference Full-text Database 6 Hits
1 Yang guangdong, Li yan'e. Shangguan Xiaoxia (Cotton research institute, Shanxi academy of agricultural science, Yuncheng 044000);Study on the Optimization of Transformation System in Chinese Cabbage[A];[C];2000
2 Zhang Fenglan Beijing Vegetable Research Centre, Beijing 100089;Transformation of Chinese cabbage by Agrobacterium-mediated Method[A];[C];2004
3 CHEN Song PU Hui-ming ZHANG Jie-fu GAO Jian-qin CHEN Feng HU Mao-long QI Cun-kou (Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Changes in the Amounts of Oleic Acid in T_o Plant Seeds of Brassica napus L. Transformed with ihpRNA Construct Targeting fad2 Gene[A];[C];2008
4 Jingqiao Wang;Genze Li;Wei Chen;Jiangming He;Yunshong Dong;Liqiong Zheng;Shouxian Cun Biotechnology Institute of Yunnan Agriculture Academy, Kunming, 650223;High-efficient transgenic platform and nonselective transformation of Brassica napus[A];[C];2003
5 Alireza Zebarjadi1,2,Mokhtar Jalali Javaran3,Ali Hatef Salmanian4,Ghasem Karimzadeh3 1 Departement of Plant Breeding and Agromoni,Faculty of Agriculture,Razi University,Kermanshah-Iran 1 Departement of Research for Biotechnology,Razi University,Kermanshah-Iran 3 Departement of Plant Breeding,Faculty of Agriculture,Tarbiat Modares University,Tehran-Iran 4 National Institute for Genetic Engineering and Biotechnology;Brassica napus plant regeneration and transformation via agrobacterium-mediated-transformation[A];[C];2007
6 Zhi Hong Yang1,Md.Jamil Hossain1,Seo Young Song 1,Hyo Yeon Lee2,Han Dae Yoon3,Yong Pyo Lim1 1Department of Horticulture,Chungnam National University,Kung-dong,Yuseong-gu,Daejeon 305-764,South Korea 2Applied Life Science,Cheju National University,Jeju 690-756,South Korea 3Division of Enviro-Biotechnology & Food Science and Technology,College of Agriculture and Life Science,Gyeongsang National University,Jinju 660-701,South Korea;An efficient Agrobacterium-mediated transformation method used in Chinese cabbage (Brassica rapa ssp. pekinensis)[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiao-dong1,2,LI Li-bin2,SONG Yi-xian3,LI Hua-yin2,LIU Li-feng2,GAO Ling1,GAO Jian-wei1,2(1.College of Life Science,Qingdao Agricultural University,Qingdao 266109,China;2.Institute of Vegetables,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Rizhao Institute of Agricultural Sciences,Juxian 276500,China);Establishment of Effective Agrobacterium-mediated Transformation System Using Chinese Cabbage Hypocotyl as Explants[J];Shandong Agricultural Sciences;2010-02
2 LIU Li-ke et al(Institute of Genetics and Developmental Biology,Chinese Academy of Science,Beijing 100101);Construction and Identification of HSP70 Antisense RNA Expression Vector for Genetic Engineering Male Sterility in Plant[J];Journal of Anhui Agricultural Sciences;2008-16
3 Wang Yanping Chen Aijun Zhang Jing Fu Shihong Xu ZhaoxiangInstitute of Virology, Chinese Academy of Preventive Medicine, Beijing;CLONING OF dsRNA ISOLATED FROM ROOT OF RAPHAN US SATJVUS(CV.XIU LI MEI ) AND DETECTON OF RA LATEDNESS OF dsRNA FROM DIFFERENT CULTIVARS BY cDNA HYBRIDIZATION ASSAY[J];Chinese Journal of Virology;1992-04
4 Yang Guangdong Li Yan'e Shi Yuejin Shang`guan Xiaoxia Wu xia;Male Sterile of Vegetable and Gene Engineering[J];Journal of Changjiang Vegetables;2001-04
5 Shen Shuxing; Liang Huifen; Zhang Chenghe; Wang Wenya; Liu Shixiong; Li Zhenqiu; Wang Dongping; Guo Yinghua (Department of Horticulture, Hebei Agricultural University, Baoding 071001);The Studies on Formation of Microspore Embryo and Plantlet in Chinese Cabbage[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-04
6 MA Qian,CHEN Xue-pin,LUO Shuang-xia,SHEN Shu-xing,WANG Yan-hua(College of Horticulture,Agricultural University of Hebei,Baoding 071001,China);Establishment of high frequency regeneration system from cotyledon in Chinese cabbage[J];Journal of Agricultural University of Hebei;2009-05
7 WANG Fu qing 1,WANG Cui lan 2,SONG Zai hua 1(1 Laiyang Agricultural College,2 Laiyang Experimental Middle School,Laiyang,Shandong 265200,China);The cytological observation of pollen and anther development in the cabbage 88-3 male sterile line[J];Acta Botanica Boreali-occidentalia Sinica;2001-03
8 MA Chun-hua~1,ZHOU Peng~(2*),WANG Hua~1 (1 College of Enology,Northwest Sci-Tech University of Agriculture and Forestrylogy,Institute of Tropical Biology Science,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China);Recombination of agrobaterium strains with trivalent-expression vectors containing chitinase/β-1,3-glucanase/ Raphanus sativus -antifingal protein genes[J];Acta Botanica Boreali-occidentalia Sinica;2004-04
9 ZHAO Jun-liang~(1,2),LU Bao-de~2,LIANG Ai-hua~1,ZHAO Mei-hua~2 (1 Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China;2 Institute of Vegetables,Shanxi Academy of Agricultural Science,Taiyuan 030031,China);Optimization of the System of Protoplast Culture and Regeneration for Chinese Cabbage[J];Acta Botanica Boreali-occidentalia Sinica;2005-03
10 XU Wei-rong,WANG Yue-jin~*,WANG Xi-ping,HAO Wei,SUN Ma(College of Horticulture,Northwest Sci-Tech University of Agriculture and Foresina Agricultural Ministry,Yangling,Shaanxi 712100,China);Construction of the Plant Expression Vectors Carrying Resistant Genes to Powdery Mildew and Adversities inWild Species of Vitis in China[J];Acta Botanica Boreali-occidentalia Sinica;2005-05
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